LOCUS       sequence-13000-e        2783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Walker SC, Avis JM, Conn GL
  TITLE     General plasmids for producing RNA in vitro transcripts with 
            homogeneous ends.
  JOURNAL   Nucleic Acids Res. 2003 Aug 1. 31(15):e82.
  PUBMED    12888534
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2003 Aug 1. 31(15):e82."
COMMENT     Sequence Label: pRZ
FEATURES             Location/Qualifiers
     source          1..2783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     411..430
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        411..429
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(562..578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(562..578)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(575..597)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    586..602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(610..616,617..634,635..640))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    655..676
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(793..810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(964..1552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1044..1063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1723..2514,2515..2583))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2346..2365
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2584..2688)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2756..2774
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc taatacgact
      421 cactataggg cccgggatcc gctagcgccg gccatggtcc cagcctcctc gctggcggcc
      481 ggtgggcaac attccgaggg gaccgtcccc tcggtaatgg cgaatgggac cctcgaggga
      541 tatctagaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      601 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
      661 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
      721 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
      781 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      841 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      901 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      961 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1081 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1141 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1201 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1261 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1321 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1381 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     1441 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1501 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1561 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1621 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1681 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     1741 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1801 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1861 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1921 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1981 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2041 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2101 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2161 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2221 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2281 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2341 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2401 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2461 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2521 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2581 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     2641 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     2701 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     2761 gcgtatcacg aggccctttc gtc
//
