LOCUS       sequence-130024-        1382 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Drosophila melanogaster Rpl10Ab-GFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Thomas A, Lee PJ, Dalton JE, Nomie KJ, Stoica L, Costa-Mattioli M, 
            Chang P, Nuzhdin S, Arbeitman MN, Dierick HA
  TITLE     A versatile method for cell-specific profiling of translated mRNAs 
            in Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e40276. doi: 10.1371/journal.pone.0040276. Epub 
            2012 Jul 6.
  PUBMED    22792260
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7):e40276. doi"; pages: " 
            10.1371/journal.pone.0040276. Epub 2012 Jul 6"
COMMENT     Sequence Label: pUAST-GFP-RpL10Ab
FEATURES             Location/Qualifiers
     source          1..1382
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..722
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(39..67)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     662..682
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcggccgcat gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
       61 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
      121 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
      181 caacacttgt cactacttta acttatggtg ttcaatgctt ttcaagatac ccagatcata
      241 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtccag gaaagaacta
      301 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
      361 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
      421 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
      481 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
      541 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
      601 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
      661 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
      721 aaatggcgtc gaaggtttcg cgtgatacgc tgtatgaggg cgtcaatgga ctcctggagg
      781 cttcggcgaa gaagaagcgc ggcttcctcg agacggtgga actgcagatc ggcctgaaga
      841 actacgatcc ccagaaggac aagcgtttct ccggcaccgt caagttgaag cacattcctc
      901 gtcccaagat gaaggtgtgc atccttggcg atcagcagca ttgcgacgaa gccaaggcca
      961 acaacgtcga cttcatggat gccgaggctc tgaagaagct gaacaagaac aagaagctgg
     1021 tgaagaagct ggccaagtcc tatgatgcct tcctggcctc tgagtcgctg atcaagcaga
     1081 ttcctcgttt gcttggccct ggcttgaaca aggccggcaa gttccctgcc ctgctgtcgc
     1141 atcaggagtc tatgattggc aagatcgagg aggttaagtc gaccatcaag ttccagatga
     1201 agaaggtgct ctgcctgtcc gtcgccgtcg gccacgttgg catgaagtcc gacgagctgg
     1261 cccaaaacgt caacttgtcg atcaacttct tggtgtcgct gttgaagaag aactggcaga
     1321 atgtgcgctc cctgcacgtc aagtcctcca tgggaccgcc ccagcgtctt tactagctcg
     1381 ag
//
