LOCUS       sequence-130026-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pUC19 vector contains a tetramethylrosamine (TMR) binding 
            riboswitch, controlling the translation initaition rate of 
            luciferase gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-TMR7pUC19
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(8..13,14..29,30..36)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     RBS             108..116
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(188..206)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 cgagctgttg acaattaatc atcggctcgt ataatgtgtg ggcaggctcc cacggatcgc
       61 gactggcgag agccaggtaa cgaatcgatc cagttccacc gattcgttaa ggaggtaatc
      121 catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatcctc tagaggatgg
      181 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
      241 tgcttttaca gatgcacata tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc
      301 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
      361 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
      421 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgaacat
      481 ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca
      541 aaaaaaatta ccaataatcc agaaaattat tatcatggat tctaaaacgg attaccaggg
      601 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga
      661 ttttgtacca gagtcctttg atcgtgacaa aacaattgca ctgataatga attcctctgg
      721 atctactggg ttacctaagg gtgtggccct tccgcataga actgcctgcg tcagattctc
      781 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtg
//
