LOCUS       sequence-130027-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pUC19 vector contains a tetramethylrosamine (TMR) binding 
            riboswitch, controlling the translation initaition rate of 
            luciferase gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-TMR7pUC19
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agcttgcatg cctgcagccg cacaccagta aggtgtgcgg ttacaatttg gactttccgc
       61 ccttcttggc ctttatgagg atctctctga tttttcttgc gtcgagtttt ccggtaagac
      121 ctttcggtac ttcgtccaca aacacaactc ctccgcgcaa ctttttcgcg gttgttactt
      181 gactggcgac gtaatccacg atctcttttt ccgtcatcgt ctttccgtgc tccaaaacaa
      241 caacggcggc gggaagttca ccggcgtcat cgtcgggaag acctgccacg cccgcgtcga
      301 agatgttggg gtgttgtaac aatatcgatt ccaattcagc gggggccacc tgatatcctt
      361 tgtatttaat taaagacttc aagcggtcaa ctatgaagaa gtgttcgtct tcgtcccagt
      421 aagctatgtc tccagaatgt agccatccat ccttgtcaat caaggcgttg gtcgcttccg
      481 gattgtttac ataaccggac ataatcatag gtcctctgac acataattcg cctctctgat
      541 taacgcccag cgttttcccg gtatccagat ccacaacctt cgcttcaaaa aatggaacaa
      601 ctttaccgac cgcgcccggt ttatcatccc cctcgggtgt aatcagaata gctgatgtag
      661 tctcagtgag cccatatcct tgtcgtatcc ctggaagatg gaagcgt
//
