LOCUS       sequence-130029-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses horse holocytochrome c in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Patel CN, Lind MC, Pielak GJ
  TITLE     Characterization of horse cytochrome c expressed in Escherichia 
            coli.
  JOURNAL   Protein Expr Purif. 2001 Jul;22(2):220-4.
  PUBMED    11437597
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2001-07"; volume: "22(2)"; pages: "220-4"
COMMENT     Sequence Label: pBTR(hCc)
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             36..58
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttccggggat cataactaac taatctagaa ataattttgt ttaactttaa gaaggagata
       61 tatccatggg cgacgtggaa aaaggcaaaa agatcttcgt gcagaaatgc gcgcagtgcc
      121 acacggtgga aaaaggcggc aaacacaaaa ccggtcccaa cctgcacggc ctgttcggcc
      181 gcaaaacggg ccaggcgccg ggcttcacgt acacggacgc gaacaaaaac aaaggcatca
      241 cgtggaaaga agaaacgctg atggaatacc tcgagaaccc gaaaaaatac atcccgggca
      301 cgaaaatgat cttcgcgggc atcaaaaaaa aaacggaacg cgaagacctg atcgcgtacc
      361 tgaaaaaggc gacgaacgaa tgataaggta ccaagatggc ctttggtggg ttgaagaagg
      421 aaaaagacag aaacgactta attacctact tgaaaaaagc cactgagtaa acaggcccct
      481 tttcctttgt cgatcggatc cggccaagct agcttttcag taattatctt tttagtaagc
      541 tagctaagtt tttacactta gttaaattgc taactttata gattacaaaa cttaggaggg
      601 tatcgatact atgaattcac aaaaaatggg ttggttttgg gcagatcaaa aaactacggg
      661 caaagatatt ggtggggcag cagtatcatc catgtcaggg tgcccagtca tgcacgagtc
      721 gtcgtcgtcg tcgccaccat cctctgagtg ccccgttatg cagggagata acgatagaat
      781 aaacccgctg aacaatatgc cggagttggc agcatccaaa cagcctggcc aaaagatgga
      841 cttgcccgtt gatcggacca tctccagcat ccccaagagt ccagacagta acgagttctg
      901 ggagtatcct tctccacaac agatgtacaa tgctatggtt agaaagggca agattggcgg
      961 tagcggcgaa
//
