LOCUS       sequence-130030-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pFTV1 vector contains a no-aptamer control mRNA encoding 
            luciferase gene. It should be used when testing AEB-Dopa5, AEB-T4-2 
            riboswitches to measure their non-specific ligand effect on 
            luciferase expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-No-aptamer-controlluciferase
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     73..91
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     RBS             172..180
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(join(200..206,207..222,223..228))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        286..388
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(455..474)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acgtgatgtt cacctcgata tgtgcatctg taaaagcaat tgttccagga accagggcgt
       61 atctcttcat agccttatgc agttgctctc cagcggttcc atcctctaga ggatagaatg
      121 gcgccgggcc tttctttatg tttttggcgt cttcgaaagc ttccattttt tacctcctta
      181 ttttttttga cgtcccacac attatacgag ccgatgatta attgtcaaca gctcggatcc
      241 cctagggaat tccgcgctcg aggatatctg gcgaaaatga gacgttgatc ggcacgtaag
      301 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc
      361 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
      421 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
      481 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
      541 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
      601 ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
      661 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
      721 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg g
//
