LOCUS       sequence-130056-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human Beta-catenin GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Han SP, Gambin Y, Gomez GA, Verma S, Giles N, Michael M, Wu SK, Guo 
            Z, Johnston W, Sierecki E, Parton RG, Alexandrov K, Yap AS
  TITLE     Cortactin scaffolds Arp2/3 and WAVE2 at the epithelial zonula 
            adherens.
  JOURNAL   J Biol Chem. 2014 Mar 14;289(11):7764-75. doi: 
            10.1074/jbc.M113.544478. Epub 2014 Jan 27.
  PUBMED    24469447
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.544478"; journalName: "J Biol Chem"; date: 
            "2014-03-14- 14"; volume: "289"; issue: "11"; pages: "7764-75"
COMMENT     Sequence Label: Human Beta-catenin GFP
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             139..174
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa
       61 gcttcgaatt ctgcagtcga cggtaccgcc accatggcta ctcaagctga tttgatggag
      121 ttggacatgg ccatggaacc agacagaaaa gcggctgtta gtcactggca gcaacagtct
      181 tacctggact ctggaatcca ttctggtgcc actaccacag ctccttctct gagtggtaaa
      241 ggcaatcctg aggaagagga tgtggatacc tcccaagtcc tgtatgagtg ggaacaggga
      301 ttttctcagt ccttcactca agaacaagta gctgatattg atggacagta tgcaatgact
      361 cgagctcaga gggtacgagc tgctatgttc cctgagacat tagatgaggg catgcagatc
      421 ccatctacac agtttgatgc tgctcatccc actaatgtcc agcgtttggc tgaaccatca
      481 cagatgctga aacatgcagt tgtaaacttg attaactatc aagatgatgc agaacttgcc
      541 acacgtgcaa tccctgaact gacaaaactg ctaaatgacg aggaccaggt ggtggttaat
      601 aaggctgcag ttatggtcca tcagctttct aaaaaggaag cttccagaca cgctatcatg
      661 cgttctcctc agatggtgtc tgctattgta cgtaccatgc agaatacaaa tgatgtagaa
      721 acagctcgtt gtaccgctgg gaccttgcat aacctttccc atcatcgtga gggcttactg
      781 gccatcttta agtctggagg cattcctgcc ctggtg
//
