LOCUS       sequence-130058-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from
            the U6 promoter and a puromycin resistance cassette and BFP from the
            EF1Alpha promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead 
            EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M,
            Weissman JS
  TITLE     Genome-Scale CRISPR-Mediated Control of Gene Repression and 
            Activation.
  JOURNAL   Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. 
            Epub 2014 Oct 9.
  PUBMED    25307932
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.09.029"; journalName: "Cell"; date: 
            "2014-10-23- 23"; volume: "159"; issue: "3"; pages: "647-61"
COMMENT     Sequence Label: pU6-sgRNA EF1Alpha-puro-T2A-BFP
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..85
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 catgacccgc aagcccggtg ccggatcggg agagggcaga ggaagtctgc taacatgcgg
       61 tgacgtcgag gagaatcctg gcccaccggt cgccaccagc gagctgatta aggagaacat
      121 gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga
      181 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg
      241 ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt
      301 catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac
      361 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      421 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa
      481 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc
      541 cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca
      601 tctgatcgca aacatcaaga ccacatatag atccaagaaa cccgctaaga acctcaagat
      661 gcctggcgtc tactatgtgg actaca
//
