LOCUS       sequence-130062-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from
            the U6 promoter and a puromycin resistance cassette and BFP from the
            EF1Alpha promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead 
            EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M,
            Weissman JS
  TITLE     Genome-Scale CRISPR-Mediated Control of Gene Repression and 
            Activation.
  JOURNAL   Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. 
            Epub 2014 Oct 9.
  PUBMED    25307932
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.09.029"; journalName: "Cell"; date: 
            "2014-10-23- 23"; volume: "159"; issue: "3"; pages: "647-61"
COMMENT     Sequence Label: pU6-sgRNA EF1Alpha-puro-T2A-BFP
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(280..313)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     317..337
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
       61 tggttagtac cgggcccgct ctagagatcc gacgcgccat ctctaggccc gcgccggccc
      121 cctcgcacgg acttgtggga gaagctcggc tactcccctg ccccggttaa tttgcatata
      181 atatttccta gtaactatag aggcttaatg tgcgataaaa gacagataat ctgttctttt
      241 taatactagc tacattttac atgataggct tggatttcta taacttcgta tagcatacat
      301 tatacgaagt tataaacagc acaaaaggaa actcacccta actgtaaagt aattgtgtgt
      361 tttgagacta taagtatccc ttggagaacc accttgttgg accaggatgg gcaccacccg
      421 tttaagagct aagctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact
      481 tgaaaaagtg gcaccgagtc ggtgcttttt ttctcgagta ctaggatcca ttaggcggcc
      541 gcgtggataa ccgtattacc gccatgcatg tgcccgtcag tgggcagagc gcacatcgcc
      601 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
      661 gcgcggggta aactg
//
