LOCUS       sequence-130069-         560 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Source of psbA promoter of Amaranthus hybridus, used as a strong 
            promoter of cyanobacterial genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Elhai J, Wolk CP
  TITLE     A versatile class of positive-selection vectors based on the 
            nonviability of palindrome-containing plasmids that allows cloning 
            into long polylinkers.
  JOURNAL   Gene. 1988 Aug 15;68(1):119-38.
  PUBMED    2851487
REFERENCE   2  (bases 1 to 560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1988-08-15"; volume: "68(1)"; pages: "119-38"
COMMENT     Sequence Label: pRL439
FEATURES             Location/Qualifiers
     source          1..560
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(49..66)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(49..65)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(58..80)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(274..293)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     393..415
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(453..471)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ccaatattca ttgagatcct ctagagtcga cctgcagcca agcttggcac tggccgtcgt
       61 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      121 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      181 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      241 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      301 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      361 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      421 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      481 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      541 cggaacccct atttgtttat
//
