LOCUS       sequence-130072-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector was used to generate a library of ca. 17-kb sequences of
            clones as part of a genomic sequence strategy and were then used to 
            complement mutations. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Kaneko T, Nakamura Y, Wolk CP, Kuritz T, Sasamoto S, Watanabe A, 
            Iriguchi M, Ishikawa A, Kawashima K, Kimura T, Kishida Y, Kohara M, 
            Matsumoto M, Matsuno A, Muraki A, Nakazaki N, Shimpo S, Sugimoto M, 
            Takazawa M, Yamada M, Yasuda M, Tabata S
  TITLE     Complete genomic sequence of the filamentous nitrogen-fixing 
            cyanobacterium Anabaena sp. strain PCC 7120.
  JOURNAL   DNA Res. 2001 Oct 31;8(5):205-13; 227-53.
  PUBMED    11759840
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Res."; 
            date: "2001-10-31"; volume: "8(5)"; pages: "205-13; 227-53"
COMMENT     Sequence Label: pRL838
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     136..153
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(315..334)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(316..334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    350..383
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
       61 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
      121 gagcggccgc atacgattta ggtgacacta taggatccct gccggccggt ccggcgcgcc
      181 cgggctcgag tgcacgtacg acgtctagac ctaggcctta agatctgatc atatgcatcc
      241 gcgggcccgg gttacgagat cgatgaattc gagctcggta cccctagagt cgacctgcag
      301 gcatgcaagc ttctccctat agtgagtcgt attagcggcc gcatcgaata taacttcgta
      361 taatgtatgc tatacgaagt tattagcgat gaggacatga ggttgccccg tattcagtgt
      421 cgctgatttg tattgtctga agttgttttt acgttaagtt gatgcagatc aattaatacg
      481 atacctgcgt cataattgat tatttgacgt ggtttgatgg cctccacgca cgttgtgata
      541 tgtagatgat aatcattatc actttacggg tcctttccgg tgatccgaca ggttacgggg
      601 cggcgacctc gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc
      661 ttcttcgtca taacttaatg tttttattta aaataccctc tgaaaagaaa ggaaacgaca
      721 ggtgctgaaa gcga
//
