LOCUS       sequence-130081-        4364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Entry vector containing the zebrafish mfap4 promoter. Used with 
            Gateway Recombination to generate transgenic constructs exhibiting 
            macrophage-restricted expression of transgenes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Walton EM, Cronan MR, Beerman RW, Tobin DM
  TITLE     The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term 
            Analysis of Macrophage Behavior during Mycobacterial Infection in 
            Zebrafish.
  JOURNAL   PLoS One. 2015 Oct 7;10(10):e0138949. doi: 
            10.1371/journal.pone.0138949. eCollection 2015.
  PUBMED    26445458
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-10-7"; volume: "10(10):e0138949. doi"; pages: " 
            10.1371/journal.pone.0138949. eCollection 2015"
COMMENT     Sequence Label: p5E-mfap4
FEATURES             Location/Qualifiers
     source          1..4364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        690..708
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2338..2354)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2338..2354)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(2391..2409)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2410..2533
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2606..2624)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2629..2645)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2629..2645)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2747..2766)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2758..3567
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2825..2844)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3714..4302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4203..4222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggt aatacgactc actatagggc gaattgggta
      721 ccgggccccc cctcgaggcg tttcttggta cagctggaaa gggaataacg cggtcagtat
      781 gaaaaccatc agcatcaaga tcaaacaagt gccttagaaa cagggctttt aaaaggaaac
      841 ctcttctctt ttaaatgtgt atctgaaaaa tcgagtctga aatgtatggc tgttggtgta
      901 atgacatttt tctctttgtt ttcactttaa agctttaata aactcctgta tgcagattta
      961 tgagattgtg tcctgctatt ctttgaaaca aaagctcatt ttccattcaa ataatgggaa
     1021 ctggattaat gtacaaaact gtaaaagggc ataaagcatt tgtggataaa gcagagtttc
     1081 catctaatga gacaaagaca acacacaata aatgcaacgc tgtggctttt gaaggtgaga
     1141 gatgctcttt gggatgctca agacagttct ggaggtaata ataataataa taatactgaa
     1201 ccactgtgat gacagacttt aaggcatttt acaataagca aaacaacatt tcagatgttt
     1261 tacagtgtgc tggaattaaa tgctgcaaag tccattgatg cgtcccatca cgcacacttt
     1321 tttctgatca tcatctgttc aaataaatca cattacatat gctgcagctt tctggattgt
     1381 ctgtagaggt ttatgggtgc aaggcaggag tccagctaga agagcattgc aatagtccag
     1441 ctttttggac taaatgttgt gcagtatgat ctcttaagaa gggtctgatg ttaaagagtg
     1501 caaacaattt ttcactgagg gattcacaca tgcagatatg tctgataatc agatcatcca
     1561 tataaattag aaatattaag aaatcgagtg gatttacagc cagattctta gtgttcgctc
     1621 ttaacttgcg ccatttgatt agctctgtca cttcaggtat tcttgtgtat gctgaatcag
     1681 tcaatgttaa cccaaagaga acactcgact ggttcttgtg ttagagaatt tagagtcttg
     1741 taaatgcaga ctccaggtta acattgcact tatctctgca ttgtgtcctt ttactcctca
     1801 accaagcttt taaccaacca atgttttata gttgttttga aaaaagtcat tagtgttgtg
     1861 caggttgaat cgtgtgtttg ttatcttctg cagaaaaggg ttatttggtg gggttatcat
     1921 aacatttgtc tgcgtttgca gatttggcag aaaacagaac cgagaactgc tgtcctttca
     1981 ctcttcggtt gtgaaaacag attgttgggt ttttgaggat tgcctcaagc aggttttgtc
     2041 acagagcacg agactcagca tcagcgattt aaaggagcgt ttgagttatt tccagctgaa
     2101 cacacacggt gagtgactta ttctgtagag cgagattata cgaagaaata acctgcaaca
     2161 ataagaaggt gtgagtgaag ctcttttaca tctcttgtag ataaactgag ctgttgagga
     2221 gagagtgaga agattgcagt aagttctgtg gctgttttat tccttgtgac gatcacattt
     2281 acacacgcca ttttgtctcg tattggtgat ttctttcttc atgactttag atcgtgggat
     2341 ccactagttc tagagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga
     2401 gggttaattc aagtttgtac aaaaaagttg aacgagaaac gtaaaatgat ataaatatca
     2461 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
     2521 tgcagtcact atgaaccaac tacttagatg gtattagtga cctgtagaat tcgagctcta
     2581 gagctgcagg gcggccgcga tatcccctat agtgagtcgt attacatggt catagctgtt
     2641 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     2701 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     2761 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
     2821 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     2881 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     2941 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     3001 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
     3061 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     3121 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     3181 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     3241 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     3301 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     3361 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     3421 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     3481 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     3541 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     3601 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     3661 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3721 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3781 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3841 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     3901 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3961 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4021 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4081 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     4141 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4201 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4261 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4321 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
