LOCUS       sequence-130102-         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB209
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(233..538)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(335..354)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tcatccttgt agtcgatgtc atgatcttta taatcaccgt catggtcttt gtagtcgctg
       61 ttatcaacca ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
      121 actattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
      181 ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac atttatattc
      241 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
      301 cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc atgcgccagc
      361 tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct gacagcagac
      421 gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta
      481 catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta aactgcattt
      541 caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg ggcgacctca
      601 gccatccctt cctgattttc cgctttccag cgtt
//
