LOCUS       sequence-130107-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pDU1-E. coli shuttle vector (Km-r) with extended polylinker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Wolk CP, Fan Q, Zhou R, Huang G, Lechno-Yossef S, Kuritz T, Wojciuch
            E
  TITLE     Paired cloning vectors for complementation of mutations in the 
            cyanobacterium Anabaena sp. strain PCC 7120.
  JOURNAL   Arch Microbiol. 2007 Dec;188(6):551-63. Epub 2007 Jul 17.
  PUBMED    17639350
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Microbiol."; date: "2007-12"; volume: "188(6)"; pages: "551-63. Epub
            2007 Jul 17"
COMMENT     Sequence Label: pRL2960
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(245..264)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(246..264)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
       61 ttggtcatga gattatcaaa aaggatccct gccggccggt ccggcgcgcc cgggctcgag
      121 tgcacgtacg acgtctagac ctaggcctta agatctgatc atatgcatcc gcgggcccgg
      181 gttacgagat cgatgaattc gagctcggta cccctagagt cgacctgcag gcatgcaagc
      241 ttctccctat agtgagtcgt attagcggcc gcaagcccaa gcgttttgtt attgggctga
      301 ctcaagatga taatgtcaag accttaggta tcgagggtga aggcggtgtc cgtaagtgtt
      361 ttaggatgtt acgcctgggt aagtttgcag ttagccacgc caagtcctta aaagaccttg
      421 cccttgtgga gtggttaaag tcggggaaat atcgctgcat ggtggatgat atgccgtgtc
      481 agttaccaga tgtaagcagt tacaaaatgg ttatgccccg gttacagtct gtaaccagtt
      541 ttcagccact ccaaacggct caaccgacct cagaacaagc attacagccc gtttcatccc
      601 tcccagaacc aagagatgaa accctagcaa aagtggtaaa agcctgtctg gaagcgggtt
      661 tttcagaaag taaggtgatt aaagaaattt tgggctatca aggcgggcgt taccaagaag
      721 gtaaatcgct actggaaaaa cttaaggatt tgtagcggtg cggtttagca ttgactgtta
      781 cttgctgtac aaggataatt ttctcaaagc ggaatggtgt taaacccgat tattagagtc
      841 gtcatagcaa a
//
