LOCUS       sequence-130135-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent On/Off aav vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Druckmann S, Feng L, Lee B, Yook C, Zhao T, Magee JC, Kim J
  TITLE     Structured Synaptic Connectivity between Hippocampal Regions.
  JOURNAL   Neuron. 2014 Feb 5;81(3):629-40. doi: 10.1016/j.neuron.2013.11.026. 
            Epub 2014 Jan 9.
  PUBMED    24412418
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.11.026"; journalName: "Neuron"; date: 
            "2014-02-5- 5"; volume: "81"; issue: "3"; pages: "629-40"
COMMENT     Sequence Label: paavCAG-Jx
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(80..113)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    132..165
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(231..251)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(649..660)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
       61 tttggcaaag aattggatca taacttcgta tagcatacat tatacgaacg gtaaagcttg
      121 atatcagatc tataacttcg tataatgtat gctatacgaa cggtacagct tatcgataat
      181 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
      241 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
      301 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
      361 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
      421 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
      481 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
      541 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
      601 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
      661 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
      721 cttcgc
//
