LOCUS       sequence-130137-         488 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to produce AAV expressing the FlpO recombinase and pig gRNA 
            towards 3 tumor suppressors: PTEN, p53 and SMAD4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Berthelsen MF, Riedel M, Cai H, Skaarup SH, Alstrup AKO, 
            Dagnaes-Hansen F, Luo Y, Jensen UB, Hager H, Liu Y, Callesen H, 
            Vendelbo MH, Jakobsen JE, Thomsen MK
  TITLE     The CRISPR/Cas9 Minipig-A Transgenic Minipig to Produce Specific 
            Mutations in Designated Tissues.
  JOURNAL   Cancers (Basel). 2021 Jun 16;13(12). pii: cancers13123024. doi: 
            10.3390/cancers13123024.
  PUBMED    34208747
REFERENCE   2  (bases 1 to 488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2021-06-16"; volume: "13(12). pii: 
            cancers13123024. doi"; pages: " 10.3390/cancers13123024"
COMMENT     Sequence Label: AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA
FEATURES             Location/Qualifiers
     source          1..488
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        47..295
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     218..237
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        316..391
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 agagggagtg gccaactcca tcactagggg ttcctgcggc cgccgagagg gcctatttcc
       61 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa
      121 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
      181 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
      241 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgcagc
      301 aattcactgt aaagcgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
      361 tcaacttgaa aaagtggcac cgagtcggtg cttttttacc ggtgagggcc tatttcccat
      421 gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt
      481 gactgtaa
//
