LOCUS       sequence-130144-        3651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modular shuttle vector for Geobacillus and E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Reeve B, Martinez-Klimova E, de Jonghe J, Leak DJ, Ellis T
  TITLE     The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile 
            Engineering.
  JOURNAL   ACS Synth Biol. 2016 Jul 1.
  PUBMED    27332993
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Jul 1."
COMMENT     Sequence Label: pG1K
FEATURES             Location/Qualifiers
     source          1..3651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(14..30)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14..30)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(27..49)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    38..54
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(62..68,69..86,87..92))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(245..262)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(416..1004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(496..515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaatgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
       61 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      121 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      181 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
      241 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      301 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      361 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      421 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      481 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      541 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      601 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      661 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      721 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      781 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      841 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      901 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      961 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1021 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1081 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1141 tctaaagtat atatgagtaa acttggtctg acagttacca atgcggccgc ctgtcgagaa
     1201 tttttggaaa atagaccttg tcgcgactcg aagattagta gtacaataga gacaacataa
     1261 cagaacggga caaaatagaa gagcttccga agcgtgtgta agtggtgctc ccaacaccgt
     1321 tcgccgtact cgccctacgg caaacacctg ctcgaaagct cttgtgttaa catatttatg
     1381 ccttgtcttc attttaatgc atagatatgt tgaatgcaag agcgaaacgc ttggaaattt
     1441 tcgtttttcc tccgatgaga gcgtgtttgc ctgcgtatta atgtacggca acgcgctctt
     1501 ttgtttgtcc cgaaaaattc gggagaaacg ggaggagaac ggaaatgtac ataatcacta
     1561 gcgaacaatt cacgcaactg aaagaaatcg agagagtcac agatttcgtt tataaacgcc
     1621 gtaagattaa aaatgagctg atggagcgta tcgccgacgc attaggccgc aagttccgca
     1681 aagcgcaaaa aacaacgcgc gtcatcgacg aaattgtttg gctcgcaaca gaccgcggtt
     1741 tttcgttccc agggcgcgaa acgcttgcca aaaagttagg cgtgtccttg ccgactgttg
     1801 atcgtgctac tcgcatgctc aaagactcgg gcgaagtcgt cgtttgctat cgtgagaatc
     1861 cgaatagtaa cggacctaaa acgcctgtat tcattttccg gagccatgca aatttcgagc
     1921 gtattgcagc cgttttaaac ttgcgtgaca acgaagctga taaagtagaa aacgccgaaa
     1981 agcctacgga atcaagcgat ttagcgcgta aaacggacgc taccattagt tcaccagttg
     2041 aaaaacatga cgatattaat aaattaatag acaatacaca tccgttggat aaaattgtaa
     2101 aatacgtcac tattaaagtg aacgaagtgc agaagcgtca tccttacggc attcgatatc
     2161 tatccgcata cataaacaaa acactcgacg acctaatgca acgtgcaaaa gcgaaggtca
     2221 aagccgaaaa agctcgcgcc caacaacgca aacaagccga ctatcaaccg accgcgaccg
     2281 gcttaatttg gtacgacttc acggcggaca caagcgccag ccagccgaca agccagcgca
     2341 agccagcgtt tgacagcgaa gacatgtttg cgccgttatt gcaggctgtt gcacgaatta
     2401 agggcgttga ggtgtgacac actcgtttta aggcgtctaa caggcgcgta gagacgtctt
     2461 taagtcgggg gaatattaaa aattccacgc ctcctaaaac gcgatatacg ccgattatga
     2521 gtttaaacga agattagatg ctataattgt tattaaaagg attgaaggat gcttaggaag
     2581 acgagttatt aatagctgaa taagaacggt gctctccaaa tattcttatt tagaaaagca
     2641 aatctaaaat tatctgaaaa gggaatgaga atagtgaatg gaccaataat aatgactaga
     2701 gaagaaagaa tgaagattgt tcatgaaatt aaggaacgaa tattggataa atatggggat
     2761 gatgttaagg ctattggtgt ttatggctct cttggtcgtc agactgatgg gccctattcg
     2821 gatattgaga tgatgtgtgt catgtcaaca gaggaagcag agttcagcca tgaatggaca
     2881 accggtgagt ggaaggtgga agtgaatttt tatagcgaag agattctact agattatgca
     2941 tctcaggtgg aatcagattg gccgcttaca catggtcaat ttttctctat tttgccgatt
     3001 tatgattcag gtggatactt agagaaagtg tatcaaactg ctaaatcggt agaagcccaa
     3061 aagttccacg atgcgatttg tgcccttatc gtagaagagc tgtttgaata tgcaggcaaa
     3121 tggcgtaata ttcgtgtgca aggaccgaca acatttctac catccttgac tgtacaggta
     3181 gcaatggcag gtgccatgtt gattggtctg catcatcgca tctgttatac gacgagcgct
     3241 tcggtcttaa ctgaagcagt taagcaatca gatcttcctt caggttatga ccatctgtgc
     3301 cagttcgtaa tgtctggtca actttccgac tctgagaaac ttctggaatc gctagagaat
     3361 ttctggaatg ggattcagga gtggacagaa cgacacggat atatagtgga tgtgtcaaaa
     3421 cgcataccat tttgataagg cgcgcctcta cggaccgagt attcaacaaa cgggccagtt
     3481 tgttgaaatc gttaattaag cgtactagtc ctaggctagc tgataccagg tcagtcccgg
     3541 gacaggtcga cctcgagtac gcctgcagga gtgttctaga ctagaagctt cactgagctc
     3601 aatttcgcgc cccgaaaggg gcgttttttt gcgaagccta aggtatcatt t
//
