LOCUS       sequence-130171-         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..116
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             137..154
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             161..589
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 atccgggacc tttaattcaa cccaacacaa tatattatag ttaaataaga attattatca
       61 aatcatttgt atattaatta aaatactata ctgtaaatta cattttattt acaatcaaag
      121 gagatatacc atggcacacc atcaccacca tcacggatcc ttggctacta cacttgaacg
      181 tattgagaag aactttgtca ttactgaccc aagattgcca gataatccca ttatattcgc
      241 gtccgatagt ttcttgcagt tgacagaata tagccgtgaa gaaattttgg gaagaaactg
      301 caggtttcta caaggtcctg aaactgatcg cgcgacagtg agaaaaatta gagatgccat
      361 agataaccaa acagaggtca ctgttcagct gattaattat acaaagagtg gtaaaaagtt
      421 ctggaacctc tttcacttgc agcctatgcg agatcagaag ggagatgtcc agtactttat
      481 tggggttcag ttggatggaa ctgagcatgt ccgagatgct gccgagagag agggagtcat
      541 gctgattaag aaaactgcag aaaatattga tgaggcggca aaagaactta tcaagtgtgt
      601 ggtggtggga gacggagctg taggtaaaac ttgcctactg atcagttaca caaccaatgc
      661 atttcctgg
//
