LOCUS       sequence-130187-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of human arginase I in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   DiCostanzo L, Sabio G, Mora A, Rodriguez PC, Ochoa AC, Centeno F, 
            Christianson DW
  TITLE     Crystal structure of human arginase I at 1.29-A resolution and 
            exploration of inhibition in the immune response.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13058-63. Epub 2005 
            Sep 2.
  PUBMED    16141327
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2005-09-13"; volume: "102(37)"; pages: 
            "13058-63. Epub 2005 Sep 2"
COMMENT     Sequence Label: HAI
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(173..277)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     345..363
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(390..395,396..412,413..418)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(593..640)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     636..654
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
       61 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
      121 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      181 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      241 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      301 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
      361 ggccctttcg tcttcaagaa ttctcatgtt tgacagctta tcatcgataa gctttaatgc
      421 ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat
      481 gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg
      541 ccggtactgc cgggcctctt gcgggatatc cggatatagt tcctcctttc agcaaaaaac
      601 ccctcaagac ccgtttagag gccccaaggg gttatgctag ttattgctca gcggtggcag
      661 cagccaactc agcttccttt cgggctttgt tagcagccgg atcccccggg ctgcaggaat
      721 tcgatatcaa gcttttaaat ttcaaaaaag ttagaatttc cacatcttga attttacacc
      781 aagagggaat ttgtagagtt tatactaatt ggaaaa
//
