LOCUS       sequence-130198-       12305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12305)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB205
FEATURES             Location/Qualifiers
     source          1..12305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(1178..1180,1181..1183,1184..1894)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1223..1244)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1484..1503)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1831..1852
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1904..2028
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2065..2070,2071..2088,2089..2095)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2149..2808
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2215..2234)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3150..3455
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3334..3353
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3496..3620)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3646..3898
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3932..3948)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3932..3948)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3945..3967)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3956..3972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3980..3986,3987..4004,4005..4010))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4025..4046
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4131..4303
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4360..4893)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(4956..5632)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    5827..5851
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6372..7163
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7409..7997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7898..7917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8151..8168
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8183..8323
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8249..8268)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8409..8431
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8667..8861
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8927..10000)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10429..11058)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg gtgagcaagg gcgaggagct
     1201 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1261 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1321 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     1381 cctgatgtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1441 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1501 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1561 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1621 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1681 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1741 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     1801 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1861 cgggatcact ctcggcatgg acgagctgta caaggctgtt atcacaagtt tgtacaaaaa
     1921 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1981 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggcccctcga
     2041 ggggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
     2101 attttgagtt aggatccggc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     2161 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     2221 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     2281 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     2341 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     2401 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     2461 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     2521 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     2581 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     2641 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     2701 atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg
     2761 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc
     2821 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
     2881 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta
     2941 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     3001 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     3061 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     3121 ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag
     3181 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     3241 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     3301 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     3361 tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     3421 aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc
     3481 agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
     3541 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
     3601 gctttcttgt acaaagtggt gataacctag agctcgaatt tccccgatcg ttcaaacatt
     3661 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     3721 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     3781 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     3841 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     3901 gaattggttc cggaaccaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3961 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     4021 cctaatgagt gagctaactc acattaggct gaattaggcg cgcctatttc tgaattcaac
     4081 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt
     4141 ttagtactgg attttggttt taggaattag attttattga tagaagtatt ttacaaatac
     4201 aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct
     4261 aattccctta tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg
     4321 actctaggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg tacttgggtc
     4381 gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag ccactgcggg
     4441 atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt tggcctcatg
     4501 cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc cggagactgc
     4561 gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc
     4621 gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg
     4681 gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc
     4741 gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag
     4801 cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc
     4861 ctgctgcgta acatcgttgc tgctgcgtac catggtcgat cgacagatct gcgaaagctc
     4921 gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat
     4981 gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc gtcatccctt
     5041 acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
     5101 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
     5161 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt
     5221 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc
     5281 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
     5341 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tcacatcaat ccacttgctt
     5401 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
     5461 tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg
     5521 gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg
     5581 gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct
     5641 ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac
     5701 catgttggca agctgctcta gccaatacgc aaaccgaatt cagaaataaa ttcagcctaa
     5761 ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
     5821 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
     5881 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg
     5941 gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc
     6001 aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa
     6061 cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct
     6121 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     6181 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     6241 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     6301 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     6361 agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     6421 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     6481 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     6541 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     6601 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     6661 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     6721 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     6781 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     6841 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     6901 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     6961 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     7021 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     7081 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     7141 tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc
     7201 gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact
     7261 atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg
     7321 gagatatatc atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7381 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7441 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7501 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     7561 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7621 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7681 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7741 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7801 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7861 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7921 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7981 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8041 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8101 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8161 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     8221 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     8281 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     8341 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     8401 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     8461 gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc
     8521 ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc
     8581 gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct
     8641 cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa
     8701 taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt
     8761 acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc
     8821 caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac
     8881 ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg
     8941 atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg
     9001 cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga
     9061 aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc
     9121 atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc
     9181 cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg
     9241 tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt
     9301 cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc
     9361 ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc
     9421 aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta
     9481 cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc
     9541 ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac
     9601 tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga
     9661 tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc
     9721 tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt
     9781 cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt
     9841 tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc
     9901 gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg
     9961 cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg
    10021 ggttccagtg ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc
    10081 cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat
    10141 gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg
    10201 gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt
    10261 acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg
    10321 gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg
    10381 cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt
    10441 tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga
    10501 ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg
    10561 ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg
    10621 cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc
    10681 aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg
    10741 ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc
    10801 ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc
    10861 aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca
    10921 atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg
    10981 cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag
    11041 tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata
    11101 tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg
    11161 cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac
    11221 aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg
    11281 tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg
    11341 ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg
    11401 cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg
    11461 tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg
    11521 cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct
    11581 gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc
    11641 gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca
    11701 cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac
    11761 gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc
    11821 gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca
    11881 tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg
    11941 tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga
    12001 cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt
    12061 tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa
    12121 agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag
    12181 acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag
    12241 tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata
    12301 aacct
//
