LOCUS       sequence-130209-         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB231
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             249..257
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 aagcacaggc tgcagggtga cggtccatcc cgctctcctg ggcacaagac atgggcagcg
       61 tgccagcagc ctgctcctcc acctccggcg ggaagccatg gctaagctta gaagccttct
      121 ccatcagggc ctccctcagc tcttttcgac taatcttgcc tgtcacgttc ctggggatag
      181 agtccacgaa ccttacgccg cccctcaggt acttggagtg agacaccctc tgggccagga
      241 agtcgtacac ctccttagcg gtgagttggg cgccgggctg cttcaccacg aagccggctg
      301 gcagctcgcc ggcctcgatg tcagggatac ccacgacggc cacgtccctg atgcctgggt
      361 gctgaaggag gatctcctcc agctccacag gggcgacctg atagcccttg tatttgatca
      421 gctccttgta ccggtccacg atgtagaaat actcgtcctc gtcgtagtag ccgaagtcgc
      481 cagagtgcag ccagccgtcg tcgtcgatgg cctccttagt agcctgtggg ttgttcacat
      541 agccttttgt taccataggt ccccagatgc acagctcgcc cacctggttt ggacccagg
//
