LOCUS       sequence-13021-e        4401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1107-HQ858671
FEATURES             Location/Qualifiers
     source          1..4401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1958..2057)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2074..2093)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2075..2093)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2098..2114)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2098..2114)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2216..2235)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2227..3036
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2294..2313)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3183..3771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3672..3691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3925..3942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4101..4128)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4220..4306)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4355..4377
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4369..4386
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4370..4386
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggagggca atgaacaggg ctgtggaggg ctgccgcaaa ggcctcccgt gctcggtgcg
      181 tccatgcaac cgccgattaa gaagctggta aggcagctcg acttcaactc ggcggcattg
      241 gcgggaaacc ccgcgatggc ggtggccgcc gccgccgtat ctagggttct gcagccgaag
      301 gcgtcgtccg tggggttcca gcatccgcag cacgtggggg ctgccatgcc gatgggagta
      361 ccgcagcagc tgcaccagcg tgtgctgccg gtgatgcgcc tgcatcaggt ggtggggcat
      421 gtgccgctgg cggtctctgt accgcagctt cgtccggtgc cgccgcagcc agtccagcgc
      481 cccccggtcg ccgttccctt gaaaccagag tcaccaaaac tacgggcaag actgtacgaa
      541 gggaaagata gtactcccac gaagaagaag tgctacaact gcaggaattc caaatgtctg
      601 aagttgtact gtgaatgctt tgcttctggt acttactgtg atggttgcaa ctgcaccaac
      661 tgttttaata atcctgataa tgaggttgcc aggcgtgaag ctgttgaagc tatattggat
      721 cgtaatccag atgctttcag gcctaagatt gggagcagcc cacatatgca taggaataat
      781 gaggtgccta gtgatctccc tttagtaggg aagcataata aagggtgcca ttgcaagaag
      841 tcaggatgtc tcaagaagta ctgtgagtgc ttccaagcta atatactatg ctctgaaaac
      901 tgcaagtgca tggattgcaa gaattttgaa ggaagtgaag agagaagaaa tcttttccaa
      961 ggggatcaca aaaatgcaat taacatgcag caggtgacaa atgcagcagt aaatggtgct
     1021 attggagcaa caggattccc ttctccttcc acatcaagga agaggaaaca taatgatcct
     1081 tcacttgacc atttcaataa ggaacatata gcgcacaaga attatcacat acctcagaaa
     1141 aatgcggtgc ctgatggttc aattccaata agccagtctg cacatcctcc aactctgggc
     1201 cccttcacct ataagcccct tttagcagac actgtccaag cagaggatat aaaggagcta
     1261 tgcaagcttt tagtggtggt atcaggagaa gctgcaaaaa catatgctgg tcagaaaacg
     1321 aaggaagaga gggtcggcga gaaggaagat gaaatcggag ggcagaagga agatgacagg
     1381 gtaggttccc tagcattgac aaaccatgat agagaaggaa ataatcaaga tcaagatcaa
     1441 aaagcaccaa ttgatgacca ttcaagcaga ggaactcata cgggcaaagc tattatagaa
     1501 gaatccagac ctaactgcgt tgatgatcat aagacccata gacctatgtc tcctggaaca
     1561 ctagcactga tgtgtgatga agaagcattt gagaaaatgt gtgacgaaca agatacaatg
     1621 tttacgactt ctcaaaatgc tgctcctcag cagacagtaa ttgtcaacca taaccaatct
     1681 gcactgtatg cagaacaaga aaacgttgtg ctgacagaat tccgggattg cctccgcaag
     1741 tttgtgagat acggaagaat gaaagaggaa aggtactcca tggcaatcaa atctgaaaca
     1801 tctggtcatc ctggacaggt aaatggtgtt tctcaagtac catattcaaa agtagatgtg
     1861 cctgctgtaa aaacatatcc acagagttcc agcagtcatc ttgttgctgg aaaacctgtt
     1921 actggccttt tggacaaaaa caagggtggg cgcgccgacc cagctttctt gtacaaagtt
     1981 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     2041 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     2101 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     2161 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     2221 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     2281 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     2341 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     2401 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     2461 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2521 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2581 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2641 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2701 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2761 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2821 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2881 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2941 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     3001 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     3061 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     3121 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3301 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3541 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     3601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3781 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3841 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3901 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3961 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4021 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     4081 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     4141 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     4201 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     4261 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     4321 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     4381 gccagtctta agctcgggcc c
//
