LOCUS       sequence-130210-         373 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB231
FEATURES             Location/Qualifiers
     source          1..373
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(147..152,153..170,171..177)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(297..316)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
       61 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggcccctc
      121 gaggggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
      181 ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa
      241 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
      301 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
      361 ttttaaagac cgt
//
