LOCUS       sequence-130211-       12479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12479)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB231
FEATURES             Location/Qualifiers
     source          1..12479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             3522..3530
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             3676..3795
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     terminator      3820..4072
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4106..4122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4106..4122)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4119..4141)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4130..4146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4154..4160,4161..4178,4179..4184))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4199..4220
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4305..4477
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4534..5067)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(5130..5806)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(5173..5193)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    6001..6025
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6546..7337
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7583..8171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8072..8091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8325..8342
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8357..8497
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8423..8442)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8583..8605
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8841..9035
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9101..10174)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10603..11232)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagca tggagagaga gaagaacgtg gtgtacggcc ccgagcccaa gcaccctctg
     2101 ggcaacttca ccgccggcga gatgctgtac aacgctctgc acaagcactc ccacatcccc
     2161 caggccatcc tggacgtgat gggcaacgag tccctttcct accaggagtt cttcgacact
     2221 actgtgaagc tgggccagag cctccagaac tgtggctaca agatgaacga tgtcgtgtcg
     2281 atctgtgcag agaacaacaa gagattcttc atccccatca tctccgcctg gtacatcggc
     2341 atggtggtgg cccctgtgaa cgaggactat atcccagacg agctgtgtaa agtgaccggc
     2401 atctccaagc cgatcctggt cttcaccact aggaagatcc tgcctaaggt tttggaggtt
     2461 aaagacagaa ccaactacat aaagaggatc atcatactgg actctgaaga gaacctgctg
     2521 ggctgcgaga gcctgcacaa cttcatgtcc aggtactccg acaacaacct ccaaacattc
     2581 aagcctctgc actacgaccc tgtggaccag gtagccgcca tcctgtgctc ctccggcaca
     2641 accggcctgc ctaaaggcgt gatgcagacc cacaggaaca tctgtgtgag actcacacac
     2701 gcatctgacc ccagagtggg tacacaactc atccccggcg tatccgtgct ggcctacctg
     2761 ccattcttcc acgccttcgg cttcagtatc aacctgggct atttcatggt gggcctgaga
     2821 gtggtgatgc tccgaaggtt taaccaggag gtgttcctga aggccatcca ggactacgag
     2881 gtgaggagcg tgatcaacgt tccctccaca atcctgttcc tgtccaagag ccctctggtg
     2941 gacaagtacg acctatccac cctggcggag ctgtgctgtg gagccgctcc tctggcgaag
     3001 gaggtggccg agatcgccgt gaagaggctg aacctgccag ggatacggtg tggctacggt
     3061 ctaacagagt ctacctccgc caacatccat actctgcaca acgagttcaa gtccggctcc
     3121 ctgggcaagg tgacacctta catggccgcc aagatcatcg acaggaacac cggcgaggcc
     3181 ctgggtccaa accaggtggg cgagctgtgc atctggggac ctatggtaac aaaaggctat
     3241 gtgaacaacc cacaggctac taaggaggcc atcgacgacg acggctggct gcactctggc
     3301 gacttcggct actacgacga ggacgagtat ttctacatcg tggaccggta caaggagctg
     3361 atcaaataca agggctatca ggtcgcccct gtggagctgg aggagatcct ccttcagcac
     3421 ccaggcatca gggacgtggc cgtcgtgggt atccctgaca tcgaggccgg cgagctgcca
     3481 gccggcttcg tggtgaagca gcccggcgcc caactcaccg ctaaggaggt gtacgacttc
     3541 ctggcccaga gggtgtctca ctccaagtac ctgaggggcg gcgtaaggtt cgtggactct
     3601 atccccagga acgtgacagg caagattagt cgaaaagagc tgagggaggc cctgatggag
     3661 aaggcttcta agcttagcca tggcttcccg ccggaggtgg aggagcaggc tgctggcacg
     3721 ctgcccatgt cttgtgccca ggagagcggg atggaccgtc accctgcagc ctgtgcttct
     3781 gctaggatca atgtgtaggc ttagagctcg aatttccccg atcgttcaaa catttggcaa
     3841 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     3901 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     3961 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     4021 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     4081 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4141 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4201 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctgaatt caacacattg
     4261 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
     4321 ctggattttg gttttaggaa ttagatttta ttgatagaag tattttacaa atacaaatac
     4381 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
     4441 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgactcta
     4501 ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc
     4561 aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc
     4621 accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag
     4681 gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc
     4741 atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag ccgcgagagc
     4801 gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa
     4861 gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt ctccgaactc
     4921 acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc cgagcctaca
     4981 tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac tgccctgctg
     5041 cgtaacatcg ttgctgctgc gtaccatggt cgatcgacag atctgcgaaa gctcgagaga
     5101 gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt
     5161 ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca
     5221 gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
     5281 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
     5341 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
     5401 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
     5461 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
     5521 ttggagtaga cgagagtgtc gtgctccacc atgttcacat caatccactt gctttgaaga
     5581 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     5641 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
     5701 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
     5761 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
     5821 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
     5881 ggcaagctgc tctagccaat acgcaaaccg aattcagaaa taaattcagc ctaattcggc
     5941 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
     6001 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
     6061 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
     6121 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
     6181 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
     6241 cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg
     6301 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     6361 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     6421 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     6481 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     6541 acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     6601 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     6661 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     6721 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     6781 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     6841 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     6901 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     6961 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     7021 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     7081 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     7141 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     7201 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     7261 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     7321 aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg
     7381 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
     7441 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
     7501 tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8281 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8341 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     8401 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     8461 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     8521 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     8581 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     8641 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     8701 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     8761 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     8821 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     8881 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     8941 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     9001 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     9061 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     9121 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     9181 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     9241 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     9301 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     9361 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
     9421 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
     9481 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
     9541 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
     9601 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
     9661 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
     9721 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
     9781 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
     9841 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
     9901 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
     9961 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
    10021 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
    10081 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
    10141 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
    10201 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
    10261 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
    10321 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
    10381 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
    10441 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
    10501 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
    10561 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
    10621 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
    10681 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
    10741 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
    10801 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
    10861 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
    10921 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
    10981 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
    11041 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
    11101 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
    11161 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
    11221 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
    11281 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
    11341 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
    11401 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
    11461 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
    11521 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
    11581 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
    11641 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
    11701 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
    11761 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
    11821 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
    11881 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
    11941 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
    12001 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
    12061 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
    12121 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
    12181 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
    12241 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
    12301 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
    12361 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
    12421 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacct
//
