LOCUS       sequence-130215-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, 
            [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains 
            bicistronially expressed cytoplasmic red fluorescent reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: BTK-APEX2-P2A-mKate2
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    110..134
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      135..144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(673..697)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcaa caagtttgta
      121 caaaaaagca ggctgccacc atggccgcag tgattctgga gagcatcttt ctgaagcgat
      181 cccaacagaa aaagaaaaca tcacctctaa acttcaagaa gcgcctgttt ctcttgaccg
      241 tgcacaaact ctcctactat gagtatgact ttgaacgtgg gagaagaggc agtaagaagg
      301 gttcaataga tgttgagaag atcacttgtg ttgaaacagt ggttcctgaa aaaaatcctc
      361 ctccagaaag acagattccg agaagaggtg aagagtccag tgaaatggag caaatttcaa
      421 tcattgaaag gttcccttat cccttccagg ttgtatatga tgaagggcct ctctacgtct
      481 tctccccaac tgaagaacta aggaagcggt ggattcacca gctcaaaaac gtaatccggt
      541 acaacagtga tctggttcag aaatatcacc cttgcttctg gatcgatggg cagtatctct
      601 gctgctctca gacagccaaa aatgctatgg gctgccaaat tttggagaac aggaatggaa
      661 gcttaaaacc ggacccagct ttcttgtaca aagtggtcga cggaaagtct tacccaactg
      721 tgagtgctga ttaccaggac gccgttgaga aggcgaagaa gaagctcaga ggcttcatcg
      781 ctgagaagag atgcgctcct ctaatgctcc gtttggcatt ccactctgct ggaacctt
//
