LOCUS       sequence-130216-         481 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB301
FEATURES             Location/Qualifiers
     source          1..481
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(108..113,114..131,132..138)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(258..277)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
       61 cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt acactttatg
      121 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta
      181 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
      241 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
      301 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
      361 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
      421 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
      481 a
//
