LOCUS       sequence-130217-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, 
            [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). 
            Also contains bicistronially expressed cytoplasmic red reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: AKT-APEX2-P2A-mKate2
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    110..134
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(634..658)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcaa caagtttgta
      121 caaaaaagca ggctgccacc atgagcgacg tggctattgt gaaggagggt tggctgcaca
      181 aacgagggga gtacatcaag acctggcggc cacgctactt cctcctcaag aatgatggca
      241 ccttcattgg ctacaaggag cggccgcagg atgtggacca acgtgaggct cccctcaaca
      301 acttctctgt ggcgcagtgc cagctgatga agacggagcg gccccggccc aacaccttca
      361 tcatccgctg cctgcagtgg accactgtca tcgaacgcac cttccatgtg gagactcctg
      421 aggagcggga ggagtggaca accgccatcc agactgtggc tgacggcctc aagaagcagg
      481 aggaggagga gatggacttc cggtcgggct cacccagtga caactcaggg gctgaagaga
      541 tggaggtgtc cctggccaag cccaagcacc gcgtgaccat gaacgagttt gagtacctga
      601 agctgctggg caagggcact ttcggcaagg tggacccagc tttcttgtac aaagtggtcg
      661 acggaaagtc ttacccaact gtgagtgctg attaccagga cgccgttgag aaggcgaaga
      721 agaagctcag aggcttcatc gctgagaaga gatgcgctcc tctaatgctc cgtttggcat
      781 tccactctgc tggaaccttt gacaagggca cgaagaccgg tggacc
//
