LOCUS       sequence-130224-       12227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12227)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB309
FEATURES             Location/Qualifiers
     source          1..12227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2050..2115
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      2134..2386
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2420..2436)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2420..2436)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2433..2455)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2444..2460
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2468..2474,2475..2492,2493..2498))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2513..2534
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    2619..2794
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(4878..5554)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(4921..4941)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    5749..5773
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6294..7085
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7331..7919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7820..7839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8073..8090
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8105..8245
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8171..8190)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8331..8353
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8589..8783
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8849..9922)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10351..10980)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagcg actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     2101 gatgacgatg acaagtagag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt
     2161 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     2221 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     2281 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     2341 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attggttccg
     2401 gaaccaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2461 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     2521 gctaactcac attaggctga attaggcgcg cctatttctg aattcaacac attgcggacg
     2581 tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt agtactggat
     2641 tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac
     2701 taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct aaattccctt
     2761 atctgggaac tactcacaca ttattatgga gaaactcgaa gttagtatat agtccttcca
     2821 tcaccctctg tgataatctc cttaaaactt gctcccccag gaatcatagg cagaacgtgc
     2881 tcctggtggg gaactatcac atccagtaaa taaggaccgg gagaatctag catagtttga
     2941 attgctgctc ttagttcatc cttcttcacc acacgcgcat ttggcacctt gaaaccgtct
     3001 gcaactgtac agaagtcggg gtagatctgc tcctcgtgat ctggatcacc taagtaagta
     3061 tgtgctctgt tggccttgta gaatctatcc tccaattgta cgaccattcc tagatgttgg
     3121 ttgtttaaga tcatgatctt cacgggtaac ttctccacaa aaattgtagc caactcctgt
     3181 acgttcatca caaagctacc atcgccatcg atgtcgatca caggaatatc tggttgagtt
     3241 gcggcagccc ccaaagcaga aggaagtccg aatcccatgg ctccaagacc tccagatgtc
     3301 aaccaacgcc gcggttcgtt gtacttgaac cactgggctg cccacatttg atgttggcca
     3361 acaccagtag atatgatggc atttccacca gtgagttcgt acagcgtctg tatggcagat
     3421 tgaggagcaa tggcatcctc gaatacggga aacttcattg gccatttctg cttttgttct
     3481 tctagttctg ccacccagtt ggagaagtcg aaagtcctct tttgactctt caagagggca
     3541 ttgatttcac ttagcgcagg tttcacatca gcacacatgg acacatgggg ctgtttgttc
     3601 tttccaattt cagcagggtc aatatcgata tgaacgatgc tggacctgct agcaaaagtc
     3661 tccagtttac cagtcactcg atcgtcgaaa cgcactccaa atgccaacaa cagatctgct
     3721 tggtccacgg cgtagttggc gtaaacagtt ccatgcattc caagcatttt caaacttaat
     3781 ggatcagact cgggatacgc tcccaaaccc attaaagtcg atgtgatagg aattccggta
     3841 agctcgacaa attccctcaa ctcttcgctg gcgtgattgc aacctcctcc agaatacact
     3901 acaggcttct tagaactaga aatgagtctt ataatctgct cgattaacgt tggttccggt
     3961 ggccgaggca gtcgttgtaa gtagcccttc agttgcattg gctgagacca atctggaata
     4021 gccatttgct gttgcacgtc cttgggaata tctaccaaga ctgggcccgg cctacctgaa
     4081 gctgccaaaa agaaagcctc cctgattacg cgagggatgt catcaatgtt cataacaagg
     4141 taattgtgct tggtgatcga tctcgtaacc tccacaattg gagtttcttg aaaagcatcg
     4201 gtaccaatca tcctactgga tacttgacct gtaatggcca ctagtggcac actgtccaag
     4261 agggcatcag cgagcccggt aacaagattg gtcgctccag gaccggatgt ggctatacaa
     4321 acccctactc gtccagacga tttagcataa ccttcagcag cgaaaatctc cccctgctcg
     4381 tggcgacaga gaatgttgcg gatgctgctg gaacgtgtaa gagcctggtg aatctccata
     4441 gacgctccgc cgggataagc gaacaccgtt ctaatccctt ctcgctcaag agcctccacc
     4501 aaaatgtcgg ctcctttgcg gggctcgtca tccttgtagc gagactgaaa gtcggacggg
     4561 gttggtgcaa cgagtgactt cccgctgcta agccgtggtc cactcacatc gactgcagac
     4621 tggatcttct cgctaacagc ttgggctttc acttccagtg aacgctcagt cttccgagag
     4681 gtgttgtatg catgtcccct cagagcggct cccgagaaga aaccactcct cacctttagc
     4741 tctttagctc ccttgcaaca agaccttgcc gatggctgca ccttctccag gctcgctatg
     4801 ctcaaaggca cagctgcgca tccactaatt gacgccatgc tcgagagaga tagatttgta
     4861 gagagagact ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag
     4921 gaagggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca
     4981 catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc
     5041 gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt
     5101 cctttatcgc aattatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg
     5161 aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga
     5221 aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg
     5281 agagtgtcgt gctccaccat gttcacatca atccacttgc tttgaagacg tggttggaac
     5341 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
     5401 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
     5461 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
     5521 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
     5581 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc
     5641 tagccaatac gcaaaccgaa ttcagaaata aattcagcct aattcggcgt taattcagta
     5701 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     5761 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     5821 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     5881 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     5941 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     6001 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     6061 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     6121 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     6181 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     6241 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
     6301 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     6361 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     6421 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     6481 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     6541 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     6601 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     6661 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     6721 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     6781 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     6841 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     6901 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     6961 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     7021 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     7081 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
     7141 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     7201 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
     7261 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7321 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7381 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7441 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     7501 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7561 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7621 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7681 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7741 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7801 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7861 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7921 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7981 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8041 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8101 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     8161 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     8221 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     8281 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     8341 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
     8401 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
     8461 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
     8521 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
     8581 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
     8641 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
     8701 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
     8761 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
     8821 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
     8881 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
     8941 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
     9001 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
     9061 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
     9121 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
     9181 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
     9241 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
     9301 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
     9361 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
     9421 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
     9481 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
     9541 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
     9601 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
     9661 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
     9721 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
     9781 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
     9841 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
     9901 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
     9961 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
    10021 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
    10081 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
    10141 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
    10201 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
    10261 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
    10321 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
    10381 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
    10441 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
    10501 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
    10561 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
    10621 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
    10681 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
    10741 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
    10801 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
    10861 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
    10921 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
    10981 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
    11041 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
    11101 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
    11161 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
    11221 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
    11281 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
    11341 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
    11401 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
    11461 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
    11521 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
    11581 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
    11641 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
    11701 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
    11761 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
    11821 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
    11881 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
    11941 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
    12001 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
    12061 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
    12121 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg
    12181 aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacct
//
