LOCUS       sequence-130225-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving APEX2-OSBP (Human OSBP domain, 
            [marks PI4P] fused to EM peroxidase marker). Also contains 
            bicistronially expressed cytoplasmic red reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: mKate2-P2A-APEX2-OSBP
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    110..134
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      135..144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcaa caagtttgta
      121 caaaaaagca ggctgccacc atggtgagcg agctgattaa ggagaacatg cacatgaagc
      181 tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca
      241 agccctacga gggcacccag accatgagaa tcaaggcggt cgagggcggc cctctcccct
      301 tcgccttcga catcctggct accagcttca tgtacggcag caaaaccttc atcaaccaca
      361 cccagggcat ccccgacttc tttaagcagt ccttccccga gggcttcaca tgggagagag
      421 tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg
      481 gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga
      541 tgcagaagaa aacactcggc tgggaggcct ccaccgagac cctgtacccc gctgacggcg
      601 gcctggaagg cagagccgac atggccctga agctcgtggg cgggggccac ctgatctgca
      661 acttgaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct
      721 actatgtgga cagaagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc
      781 agcacgaggt ggctgtggcc agatactgcg acctcc
//
