LOCUS       sequence-130242-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of CENP-T  aa 1-242  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKG212
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             287..307
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             320..364
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
       61 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      121 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
      181 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
      241 gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
      301 tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      361 cagcggaggt ggaggtacta gtctcgagat ggcagaccac aaccctgaca gcgattctac
      421 acctcgtaca ctgctgcgtc gtgtgctgga tactgctgat cctcgtactc ctcgtcgtcc
      481 tcgtagcgct cgtgctggtg ctcgccgtgc cctgctggaa acagcctctc ctcgtaaact
      541 gagtggccag acccgtacta ttgcccgtgg gcgttcacat ggagcccgct cggtcggtcg
      601 tagtgctcac attcaagcct cgggacacct ggaagaacaa acccctcgta ccctgctgaa
      661 aaacatcctg ctgactgccc ctgaaagctc tattctgatg ccggaaagcg tcgttaaacc
      721 ggttcctgct cctcaagccg ttcaaccttc tcgtcaagag tcctcctgtg gatctctgga
      781 gctgcaactg cctgaactgg agcctcctac aacactggca ccggggctgc tggcgcctgg
      841 tcgtcgtaa
//
