LOCUS       sequence-130248-         439 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB309
FEATURES             Location/Qualifiers
     source          1..439
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(228..293)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    304..428
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
       61 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
      121 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
      181 aaactttatt gccaaatgtt tgaacgatcg gggaaattcg agctctactt gtcatcgtca
      241 tccttgtagt cgatgtcatg atctttataa tcaccgtcat ggtctttgta gtcgctgtta
      301 tcaaccactt tgtacaagaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
      361 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
      421 tcactatggt cgacctgca
//
