LOCUS       sequence-130251-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone containing APEX2 with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: pME-APEX2-NS
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      78..87
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt
       61 gtacaaaaaa gcaggctgcc accatgggaa agtcttaccc aactgtgagt gctgattacc
      121 aggacgccgt tgagaaggcg aagaagaagc tcagaggctt catcgctgag aagagatgcg
      181 ctcctctaat gctccgtttg gcattccact ctgctggaac ctttgacaag ggcacgaaga
      241 ccggtggacc cttcggaacc atcaagcacc ctgccgaact ggctcacagc gctaacaacg
      301 gtcttgacat cgctgttagg cttttggagc cactcaaggc ggagttccct attttgagct
      361 acgccgattt ctaccagttg gctggcgttg ttgccgttga ggtcacgggt ggacctaagg
      421 ttccattcca ccctggaaga gaggacaagc ctgagccacc accagagggt cgcttgcccg
      481 atcccactaa gggttctgac catttgagag atgtgtttgg caaagctatg gggcttactg
      541 accaagatat cgttgctcta tctgggggtc acactattgg agctgcacac aaggagcgtt
      601 ctggatttga gggtccctgg acctctaatc ctcttatttt cgacaactca tacttcacgg
      661 agttgttgag tggtgagaag gaaggtctcc ttcagctacc ttctgacaag gctcttttgt
      721 ctgaccctgt attccgccct ctcgttgaca aatatgcagc ggacgaagat gccttctttg
      781 ctgattacgc tgaggctcac caa
//
