LOCUS       sequence-130257-         473 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB320
FEATURES             Location/Qualifiers
     source          1..473
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(107..112,113..130,131..137)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(257..276)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
       61 agtcactatg gcggcccctc gaggggccgc attaggcacc ccaggcttta cactttatgc
      121 ttccggctcg tataatgtgt ggattttgag ttaggatccg tctagatttt caggagctaa
      181 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
      241 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      301 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      361 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
      421 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttt
//
