LOCUS       sequence-130258-       13079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13079)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB320
FEATURES             Location/Qualifiers
     source          1..13079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2986..3238
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3272..3288)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3272..3288)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3285..3307)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3296..3312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3320..3326,3327..3344,3345..3350))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3365..3386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3471..3646
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(5730..6406)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(5773..5793)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    6601..6625
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7146..7937
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8183..8771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8672..8691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8925..8942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8957..9097
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9023..9042)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9183..9205
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9441..9635
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9701..10774)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11203..11832)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtct
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagct tggatttgga tttggagttg cgcttgggat tcgcctccgc cggaatggct
     2101 agtgaagctc gaaaaacaaa gaaaaaaatc aaagggattc agcaagccac tgcaggagtc
     2161 tcacaagaca cttcggaaaa tcctaacaaa acaatagttc ctgcagcatt accacagctc
     2221 acccctacct tggtgtcact gctggaggtg attgaacccg aggtgttgta tgcaggatat
     2281 gatagctctg ttccagattc agcatggaga attatgacca cactcaacat gttaggtggg
     2341 cgtcaagtga ttgcagcagt gaaatgggca aaggcgatac caggcttcag aaacttacac
     2401 ctggatgacc aaatgaccct gctacagtac tcatggatgt ttctcatggc atttgccctg
     2461 ggttggagat catacagaca atcaagtgga aacctgctct gctttgctcc tgatctgatt
     2521 attaatgagc agagaatgtc tctaccctgc atgtatgacc aatgtaaaca catgctgttt
     2581 gtctcctctg aattacaaag attgcaggta tcctatgaag agtatctctg tatgaaaacc
     2641 ttactgcttc tctcctcagt tcctaaggaa ggtctgaaga gccaagagtt atttgatgag
     2701 attcgaatga cttatatcaa agagctagga aaagccatcg tcaaaaggga agggaactcc
     2761 agtcagaact ggcaacggtt ttaccaactg acaaaacttc tggactccat gcatgaggtg
     2821 gttgagaatc tccttaccta ctgcttccag acatttttgg ataagaccat gagtattgaa
     2881 ttcccagaga tgttagctga aatcatcact aatcagatac caaaatattc aaatggaaat
     2941 atcaagaagc tgctgtttca tcaaaaatag agctcgaatt tccccgatcg ttcaaacatt
     3001 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     3061 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     3121 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     3181 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     3241 gaattggttc cggaaccaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3301 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3361 cctaatgagt gagctaactc acattaggct gaattaggcg cgcctatttc tgaattcaac
     3421 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt
     3481 ttagtactgg attttggttt taggaattag aaattttatt gatagaagta ttttacaaat
     3541 acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc
     3601 ctaaattccc ttatctggga actactcaca cattattatg gagaaactcg aagttagtat
     3661 atagtccttc catcaccctc tgtgataatc tccttaaaac ttgctccccc aggaatcata
     3721 ggcagaacgt gctcctggtg gggaactatc acatccagta aataaggacc gggagaatct
     3781 agcatagttt gaattgctgc tcttagttca tccttcttca ccacacgcgc atttggcacc
     3841 ttgaaaccgt ctgcaactgt acagaagtcg gggtagatct gctcctcgtg atctggatca
     3901 cctaagtaag tatgtgctct gttggccttg tagaatctat cctccaattg tacgaccatt
     3961 cctagatgtt ggttgtttaa gatcatgatc ttcacgggta acttctccac aaaaattgta
     4021 gccaactcct gtacgttcat cacaaagcta ccatcgccat cgatgtcgat cacaggaata
     4081 tctggttgag ttgcggcagc ccccaaagca gaaggaagtc cgaatcccat ggctccaaga
     4141 cctccagatg tcaaccaacg ccgcggttcg ttgtacttga accactgggc tgcccacatt
     4201 tgatgttggc caacaccagt agatatgatg gcatttccac cagtgagttc gtacagcgtc
     4261 tgtatggcag attgaggagc aatggcatcc tcgaatacgg gaaacttcat tggccatttc
     4321 tgcttttgtt cttctagttc tgccacccag ttggagaagt cgaaagtcct cttttgactc
     4381 ttcaagaggg cattgatttc acttagcgca ggtttcacat cagcacacat ggacacatgg
     4441 ggctgtttgt tctttccaat ttcagcaggg tcaatatcga tatgaacgat gctggacctg
     4501 ctagcaaaag tctccagttt accagtcact cgatcgtcga aacgcactcc aaatgccaac
     4561 aacagatctg cttggtccac ggcgtagttg gcgtaaacag ttccatgcat tccaagcatt
     4621 ttcaaactta atggatcaga ctcgggatac gctcccaaac ccattaaagt cgatgtgata
     4681 ggaattccgg taagctcgac aaattccctc aactcttcgc tggcgtgatt gcaacctcct
     4741 ccagaataca ctacaggctt cttagaacta gaaatgagtc ttataatctg ctcgattaac
     4801 gttggttccg gtggccgagg cagtcgttgt aagtagccct tcagttgcat tggctgagac
     4861 caatctggaa tagccatttg ctgttgcacg tccttgggaa tatctaccaa gactgggccc
     4921 ggcctacctg aagctgccaa aaagaaagcc tccctgatta cgcgagggat gtcatcaatg
     4981 ttcataacaa ggtaattgtg cttggtgatc gatctcgtaa cctccacaat tggagtttct
     5041 tgaaaagcat cggtaccaat catcctactg gatacttgac ctgtaatggc cactagtggc
     5101 acactgtcca agagggcatc agcgagcccg gtaacaagat tggtcgctcc aggaccggat
     5161 gtggctatac aaacccctac tcgtccagac gatttagcat aaccttcagc agcgaaaatc
     5221 tccccctgct cgtggcgaca gagaatgttg cggatgctgc tggaacgtgt aagagcctgg
     5281 tgaatctcca tagacgctcc gccgggataa gcgaacaccg ttctaatccc ttctcgctca
     5341 agagcctcca ccaaaatgtc ggctcctttg cggggctcgt catccttgta gcgagactga
     5401 aagtcggacg gggttggtgc aacgagtgac ttcccgctgc taagccgtgg tccactcaca
     5461 tcgactgcag actggatctt ctcgctaaca gcttgggctt tcacttccag tgaacgctca
     5521 gtcttccgag aggtgttgta tgcatgtccc ctcagagcgg ctcccgagaa gaaaccactc
     5581 ctcaccttta gctctttagc tcccttgcaa caagaccttg ccgatggctg caccttctcc
     5641 aggctcgcta tgctcaaagg cacagctgcg catccactaa ttgacgccat gctcgagaga
     5701 gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt
     5761 ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca
     5821 gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
     5881 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
     5941 acgatagcct ttcctttatc gcaattatgg catttgtagg tgccaccttc cttttctact
     6001 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
     6061 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
     6121 ttggagtaga cgagagtgtc gtgctccacc atgttcacat caatccactt gctttgaaga
     6181 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     6241 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
     6301 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
     6361 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
     6421 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
     6481 ggcaagctgc tctagccaat acgcaaaccg aattcagaaa taaattcagc ctaattcggc
     6541 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
     6601 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
     6661 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
     6721 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
     6781 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
     6841 cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg
     6901 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     6961 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     7021 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     7081 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     7141 acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     7201 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     7261 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     7321 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     7381 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     7441 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     7501 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     7561 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     7621 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     7681 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     7741 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     7801 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     7861 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     7921 aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg
     7981 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
     8041 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
     8101 tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8161 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8221 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8281 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     8341 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8401 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8521 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8581 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8701 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8761 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8821 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8881 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8941 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     9001 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     9061 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     9121 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     9181 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     9241 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     9301 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     9361 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     9421 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     9481 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     9541 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     9601 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     9661 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     9721 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     9781 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     9841 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     9901 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     9961 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
    10021 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
    10081 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
    10141 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
    10201 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
    10261 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
    10321 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
    10381 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
    10441 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
    10501 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
    10561 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
    10621 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
    10681 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
    10741 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
    10801 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
    10861 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
    10921 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
    10981 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
    11041 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
    11101 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
    11161 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
    11221 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
    11281 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
    11341 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
    11401 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
    11461 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
    11521 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
    11581 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
    11641 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
    11701 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
    11761 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
    11821 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
    11881 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
    11941 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
    12001 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
    12061 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
    12121 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
    12181 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
    12241 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
    12301 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
    12361 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
    12421 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
    12481 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
    12541 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
    12601 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
    12661 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
    12721 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
    12781 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
    12841 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
    12901 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
    12961 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
    13021 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacct
//
