LOCUS       sequence-130261-        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pCVL Traffic Light Reporter 1.1 (Sce target) E...
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      134..143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(185..206)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(467..486)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     814..835
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             892..945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(1088..1106)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
       61 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccgga
      121 tcgatcctcg agcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      181 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcctcga
      241 cattaccctg ttatccctac gcaaataaga gctcacctac ggcaagctga ccctgaagtt
      301 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
      361 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      421 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      481 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      541 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
      601 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
      661 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
      721 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
      781 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
      841 cgccgggatc actctcggca tggacgagct gtacaagtaa ctggatccgg tgagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatccgg gccctgtgag caagggcgag
      961 gaggataact ccgccatcat caaggagttc ctgcgcttca aggtgcacat ggagggctcc
     1021 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     1081 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg gga
//
