LOCUS       sequence-130266-        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pCVL Traffic Light Reporter 1.1 (Sce target) E...
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(230..937)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNSAIIKEFLRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(508..527)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     777..795
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(938..991)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact actgcactta tatacggttc
       61 tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca tcactttccc agtttacccc
      121 gcgccacctt ctctaggcac cggttcaatt gccgacccct ccccccaact tctcggggac
      181 tgtgggcgat gtgcgctctg cccactgacg ggcaccggag cctctgcatt cacttgtaca
      241 gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg
      301 tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca
      361 gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt
      421 ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc
      481 gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga
      541 agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcaga gaggagtcct
      601 gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct
      661 cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg
      721 agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccacccttgg
      781 tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga
      841 tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc aggaactcct
      901 tgatgatggc ggagttatcc tcctcgccct tgctcacagg gcccggattc tcctccacgt
      961 caccgcatgt tagaagactt cctctgccct caccggatcc agttacttgt acagctcgtc
     1021 catgccgaga gtgatcc
//
