LOCUS       sequence-13027-e        3471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1133-HQ858677
FEATURES             Location/Qualifiers
     source          1..3471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      119..128
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1028..1127)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1144..1163)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1145..1163)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1168..1184)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1168..1184)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1286..1305)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1297..2106
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1364..1383)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2253..2841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2742..2761
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2995..3012
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3171..3198)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3290..3376)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3425..3447
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3439..3456
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3440..3456
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggcggccg tgcagcagca gcagcagcag cagcgccgcg acgcggaggc ggagctcaac
      181 ctgcccccgg gcttccggtt ccacccgacg gacgaggagc tggtggcgca ctacctctgc
      241 gcgcgcgccg cggggcgccg cccgccggtg tccatcatcg ccgaggtcga cctgtaccgc
      301 ttcgacccct gggacctgcc ggagcgcgcc ctcttcgggc gccgcgagtg gtacttcttc
      361 acgccgcggg accgcaagta ccccaacggc tcccgcccca accgcgccgc cgggtcgggc
      421 tactggaagg ccacgggcgc ggacaagccc gtggagcacg agggccggac ggcggggatc
      481 aagaaggcgc tcgtgttcta ccacggcaag ccgccgcgcg gggtcaagac ggagtggatc
      541 atgcacgagt accgcctcgc cgaagccggc ggcgcccgcg ccaagaagtc cggcgccggc
      601 acgctcaggc tggatgactg ggtgctgtgc cgcctgtaca acaagaagaa cgagtgggag
      661 aagatgcagc agcagaagga gaaggagaag aaggcgatgg agtcggaggc gtcgctctcg
      721 cactcccact ccgacacgcg gacgccggaa tcggagatcg acgacgaccc gttcccggag
      781 ctgggctctc taccggcgtt cgacgacatg ggcgcagccc cagcccctgc cggcgtcgtc
      841 ctgcccaagg aggaggtgga ggacttcggc gacctcggcg gcgacgactg gctcgcgggc
      901 atcaacctcg acgacctgca gatgccgggg gacgccgccg atttctacgg ctccatgctc
      961 gtctcgccga tggccgccaa gatggagccg gacggcggct tccccttctt caagggtggg
     1021 cgcgccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     1081 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     1141 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     1201 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1261 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1321 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1381 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     1441 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     1501 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     1561 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     1621 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     1681 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     1741 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     1801 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     1861 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     1921 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     1981 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2041 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     2101 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     2161 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     2221 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2281 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2341 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     2401 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2461 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2521 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2581 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2641 gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2701 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2761 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2821 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2881 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     2941 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3001 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3061 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3121 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     3181 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     3241 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     3301 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     3361 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     3421 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc c
//
