LOCUS       sequence-130277-         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB426
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..152
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(193..498)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(295..314)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ccaccggagc ctccagcgct gttatcaacc actttgtaca agaaagctga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag
      181 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc
      241 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc
      301 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga
      361 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt
      421 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt
      481 gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt
      541 caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac
//
