LOCUS       sequence-13028-e        4638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1134-HQ858678
FEATURES             Location/Qualifiers
     source          1..4638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      119..128
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2195..2294)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2311..2330)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2312..2330)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2335..2351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2335..2351)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2453..2472)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2464..3273
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2531..2550)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3420..4008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3909..3928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4162..4179
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4338..4365)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4457..4543)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4592..4614
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4606..4623
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4607..4623
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggcggcgg caaaggcgcg gggaggggag ttccccgtgg gcatgaaggt gctggttgtg
      181 gacgacgacc cgacgtgcct cgttgtgctc aagaggatgc tccttgagtg ccgatatgac
      241 gtgacaacat gtcctcaggc tacaagagca ctaactatgt tgcgagagaa taggcgtggt
      301 tttgatgtta taataagtga tgtccacatg ccggatatgg acggattcag gctacttgaa
      361 cttgtaggcc ttgagatgga ccttccagtt atcatgatgt ctgctgattc aagaacggat
      421 attgtaatga acggaattaa gcatggagca tgtgactatt taataaaacc tgtcagaatg
      481 gaggagctga aaaacatctg gcaacatgtt attaggaaaa aatttaatga aaacaaggac
      541 catgagcatt ctggtagcct agatgacacc gatcgtaaca gaccaaccaa taatgataat
      601 gaatacgctt cctccgcgaa tgatggaggt gatggcagct ggaaatctca gagaaagaaa
      661 agagagaaag aagatgatga aactgacctc gaaaatggtg atccttcttc tacatcaaag
      721 aaaccaagag ttgtttggtc agttgagctt catcaacaat ttgtgaatgc agtcaatcac
      781 ctcgggatag acaaagctgt cccaaagaaa attttggaat tgatgaatgt cccaggctta
      841 accagggaaa atgttgccag ccatttgcag aaattcagac tctacctgaa gagaattgct
      901 cagcatcatg caggaatacc tcatccattt gttgcgcctg tatctagtgc taacgttgct
      961 ccgttaggag gactggaatt ccaagctttg gctgcttctg gtcagatccc tcctcaagct
     1021 ctggctgctt tgcaggatga actccttggt cgacctacaa gcagtttggc gttgcctgga
     1081 agggaccagt catctttgcg agtggctgca atcaaaggaa acaagcacca tgaagaaaga
     1141 gaaatagcat ttggtcaacc catatacaag tgtcagaata atgcatatgg tgcattccct
     1201 caaaacagcc cagcagttgg aggattgcaa ccttttgcag cttggcccaa taacaaagtt
     1261 ggtatgactg attcaacaag cacattggga aatgtgggca attctcaaaa tagcaatatg
     1321 ctattgcatg aattgcagca acagccagac accttgctgt taggaaccct tcacaatatt
     1381 gatgccaaac cttctggtgt agttatgcca tctcagtcgt taaatacatt cccggctagt
     1441 gagggtatct cacctaatca aaatcccttg attataccat ctcaaccccc aagttttgtg
     1501 tcatcaattc ctccatccat gaaacatgaa tctcttcttg gattaccttc aacgtcaacc
     1561 agtctgttgg gcgggcttga tatggttaat caagcttcaa caagtcaggc tttgattagt
     1621 agccatggaa caaatcttcc tggtctcatg aaccgtagct caaatgcaat cccttcacca
     1681 ggaattagta attttcaaag tggaaatatt cattatgttg ttaatcagaa cgctatggga
     1741 gttagctcta agccaccagg tgttctaaag accgagagca ctgactcact gagttgtagt
     1801 tatggctata ttggtggtag caccagtgtg gactctggct tgttctcttc tcagtccaaa
     1861 aatccacagt atgatctact gcagaatcaa aatgatgtta acggcagctg gtcgccttca
     1921 caagattttg atagttttgg aaattctctt gggcaaggcc accctggtac cacttcatct
     1981 aacttccaga gttccgccct tgggaagttg cctgaccaag gacgagggag aaatcatggg
     2041 tttgtcggga aaggcacttg cattccaagc cgctttgcag tggatgaggt tgaatctcca
     2101 actaacaact tgagccacag cattggaaac agtggagaca tagtgaaccc cgacatattt
     2161 ggatttagtg gacatatgaa gggtgggcgc gccgacccag ctttcttgta caaagttggc
     2221 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     2281 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     2341 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     2401 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2461 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     2521 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2581 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2641 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2701 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2761 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2821 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2881 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2941 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     3001 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     3061 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     3121 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     3181 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     3241 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     3301 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     3361 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3421 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3481 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3541 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3601 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3661 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3721 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3781 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     3841 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3901 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3961 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4021 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4081 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4141 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     4201 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     4261 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     4321 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     4381 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4441 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4501 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4561 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4621 agtcttaagc tcgggccc
//
