LOCUS       sequence-130280-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Human CDK8 Wild-Type.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Pelish HE, Liau BB, Nitulescu II, Tangpeerachaikul A, Poss ZC, Da 
            Silva DH, Caruso BT, Arefolov A, Fadeyi O, Christie AL, Du K, Banka 
            D, Schneider EV, Jestel A, Zou G, Si C, Ebmeier CC, Bronson RT, 
            Krivtsov AV, Myers AG, Kohl NE, Kung AL, Armstrong SA, Lemieux ME, 
            Taatjes DJ, Shair MD
  TITLE     Mediator kinase inhibition further activates 
            super-enhancer-associated genes in AML.
  JOURNAL   Nature. 2015 Sep 28. doi: 10.1038/nature14904.
  PUBMED    26416749
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Sep 28. doi: 10.1038/nature14904."
COMMENT     Sequence Label: pLVX-Flag-CDK8-WT-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             47..70
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 catttcaggt gtcgtgagga tctatttccg gtgattcgcc accatggact acaaagacga
       61 tgacgacaag agatctatgg actatgactt taaagtgaag ctgagcagcg agcgggagcg
      121 ggtcgaggac ctgtttgaat acgagggctg caaagttggc cgaggcactt atggtcacgt
      181 ctacaaagcc aagaggaaag atgggaagga tgataaagac tatgctttaa aacaaataga
      241 aggaactggg atctctatgt cggcatgtag agaaatagca ttacttcgag agcttaagca
      301 tccaaacgtc atttctcttc aaaaggtgtt tctgtctcat gctgatagga aggtgtggct
      361 tctgtttgac tatgctgaac atgacctctg gcatataatc aagtttcaca gagcttctaa
      421 agcaaacaag aagccagttc agttacctcg gggaatggtg aagtcactat tatatcagat
      481 cctagatggt attcactacc tgcatgctaa ctgggtgttg cacagagatt tgaaacctgc
      541 taatatttta gttatgggtg aaggtcctga gcgaggaaga gtaaaaattg ctgacatggg
      601 ctttgcccga ttatttaatt cacctttgaa gcctttagca gatttggatc cagtggttgt
      661 tacattctgg taccgagccc ctgaactact tcttggagca aggcattata ccaaagctat
      721 tgatatttgg gctatagggt gtatatttgc agaactacta acgtcagaac caatatttca
      781 ctgtcgacaa gaggacatca aaactagtaa tccttatcac catgaccagc tggacagaat
      841 attcaatgta atgggatttc ctgcagataa agattgggaa gatataaaaa agatgcctga
      901 acattcaaca ttaatgaaag atttcagaag aaatacgtat accaactgca gccttatcaa
      961 gtatatggaa aaa
//
