LOCUS       sequence-130290-         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB431
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..118)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARIN"
     RBS             264..272
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cattgatcct agcagaagca caggctgcag ggtgacggtc catcccgctc tcctgggcac
       61 aagacatggg cagcgtgcca gcagcctgct cctccacctc cggcgggaag ccatggctaa
      121 gcttagaagc cttctccatc agggcctccc tcagctcttt tcgactaatc ttgcctgtca
      181 cgttcctggg gatagagtcc acgaacctta cgccgcccct caggtacttg gagtgagaca
      241 ccctctgggc caggaagtcg tacacctcct tagcggtgag ttgggcgccg ggctgcttca
      301 ccacgaagcc ggctggcagc tcgccggcct cgatgtcagg gatacccacg acggccacgt
      361 ccctgatgcc tgggtgctga aggaggatct cctccagctc cacaggggcg acctgatagc
      421 ccttgtattt gatcagctcc ttgtaccggt ccacgatgta gaaatactcg tcctcgtcgt
      481 agtagccgaa gtcgccagag tgcagccagc cgtcgtcgtc gatggcctcc ttagtagcct
      541 gtgggttgtt cacatagcct tttgttacca taggtcccca gatgcacagc tcgcccacct
      601 ggtttggacc c
//
