LOCUS       sequence-130291-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Human CDK8 W105M Mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Pelish HE, Liau BB, Nitulescu II, Tangpeerachaikul A, Poss ZC, Da 
            Silva DH, Caruso BT, Arefolov A, Fadeyi O, Christie AL, Du K, Banka 
            D, Schneider EV, Jestel A, Zou G, Si C, Ebmeier CC, Bronson RT, 
            Krivtsov AV, Myers AG, Kohl NE, Kung AL, Armstrong SA, Lemieux ME, 
            Taatjes DJ, Shair MD
  TITLE     Mediator kinase inhibition further activates 
            super-enhancer-associated genes in AML.
  JOURNAL   Nature. 2015 Sep 28. doi: 10.1038/nature14904.
  PUBMED    26416749
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Sep 28. doi: 10.1038/nature14904."
COMMENT     Sequence Label: pLVX-Flag-CDK8-W105M-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             45..68
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tttcaggtgt cgtgaggatc tatttccggt gattcgccac catggactac aaagacgatg
       61 acgacaagag atctatggac tatgacttta aagtgaagct gagcagcgag cgggagcggg
      121 tcgaggacct gtttgaatac gagggctgca aagttggccg aggcacttat ggtcacgtct
      181 acaaagccaa gaggaaagat gggaaggatg ataaagacta tgctttaaaa caaatagaag
      241 gaactgggat ctctatgtcg gcatgtagag aaatagcatt acttcgagag cttaagcatc
      301 caaacgtcat ttctcttcaa aaggtgtttc tgtctcatgc tgataggaag gtgtggcttc
      361 tgtttgacta tgctgaacat gacctcatgc atataatcaa gtttcacaga gcttctaaag
      421 caaacaagaa gccagttcag ttacctcggg gaatggtgaa gtcactatta tatcagatcc
      481 tagatggtat tcactacctg catgctaact gggtgttgca cagagatttg aaacctgcta
      541 atattttagt tatgggtgaa ggtcctgagc gaggaagagt aaaaattgct gacatgggct
      601 ttgcccgatt atttaattca cctttgaagc ctttagcaga tttggatcca gtggttgtta
      661 cattctggta ccgagcccct gaactacttc ttggagcaag gcattatacc aaagctattg
      721 atatttgggc tatagggtgt atatttgcag aactactaac gtcagaacca atatttcact
      781 gtcgacaaga ggacatcaaa actagtaatc cttatcacca tgaccagctg gacagaatat
      841 tcaatgtaat gggatttcct gcagataaag attgggaaga tataaaaaa
//
