LOCUS       sequence-130295-       10325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Human CDK8 W105M Mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Pelish HE, Liau BB, Nitulescu II, Tangpeerachaikul A, Poss ZC, Da 
            Silva DH, Caruso BT, Arefolov A, Fadeyi O, Christie AL, Du K, Banka 
            D, Schneider EV, Jestel A, Zou G, Si C, Ebmeier CC, Bronson RT, 
            Krivtsov AV, Myers AG, Kohl NE, Kung AL, Armstrong SA, Lemieux ME, 
            Taatjes DJ, Shair MD
  TITLE     Mediator kinase inhibition further activates 
            super-enhancer-associated genes in AML.
  JOURNAL   Nature. 2015 Sep 28. doi: 10.1038/nature14904.
  PUBMED    26416749
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Sep 28. doi: 10.1038/nature14904."
COMMENT     Sequence Label: pLVX-Flag-CDK8-W105M-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..10325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2337..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3540..3549
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3549..3572
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4996..5568
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5019..5570
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5163..5180)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             5570..6280
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5715..5733)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5983..6002
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6294..6882
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6347..6367)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6765..6776)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7089..7722
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7850..7866)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7850..7866)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7863..7885)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7874..7890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7898..7904,7905..7922,7923..7928))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7943..7964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8081..8098)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8252..8840)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8332..8351)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9011..9802,9803..9871))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9634..9653
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9872..9976)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10024..10158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10061..10080)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10115..10134
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgattcg
     3541 ccaccatgga ctacaaagac gatgacgaca agagatctat ggactatgac tttaaagtga
     3601 agctgagcag cgagcgggag cgggtcgagg acctgtttga atacgagggc tgcaaagttg
     3661 gccgaggcac ttatggtcac gtctacaaag ccaagaggaa agatgggaag gatgataaag
     3721 actatgcttt aaaacaaata gaaggaactg ggatctctat gtcggcatgt agagaaatag
     3781 cattacttcg agagcttaag catccaaacg tcatttctct tcaaaaggtg tttctgtctc
     3841 atgctgatag gaaggtgtgg cttctgtttg actatgctga acatgacctc atgcatataa
     3901 tcaagtttca cagagcttct aaagcaaaca agaagccagt tcagttacct cggggaatgg
     3961 tgaagtcact attatatcag atcctagatg gtattcacta cctgcatgct aactgggtgt
     4021 tgcacagaga tttgaaacct gctaatattt tagttatggg tgaaggtcct gagcgaggaa
     4081 gagtaaaaat tgctgacatg ggctttgccc gattatttaa ttcacctttg aagcctttag
     4141 cagatttgga tccagtggtt gttacattct ggtaccgagc ccctgaacta cttcttggag
     4201 caaggcatta taccaaagct attgatattt gggctatagg gtgtatattt gcagaactac
     4261 taacgtcaga accaatattt cactgtcgac aagaggacat caaaactagt aatccttatc
     4321 accatgacca gctggacaga atattcaatg taatgggatt tcctgcagat aaagattggg
     4381 aagatataaa aaagatgcct gaacattcaa cattaatgaa agatttcaga agaaatacgt
     4441 ataccaactg cagccttatc aagtatatgg aaaaacataa agttaaacca gatagtaaag
     4501 cattccactt gcttcagaag ctgcttacca tggacccaat aaagcgaatt acctcagaac
     4561 aggctatgca ggacccctat ttcttagaag acccacttcc tacatcagac gtttttgccg
     4621 gttgtcaaat cccttaccca aaacgagaat ttttaacgga agaagaacct gatgacaaag
     4681 gagacaaaaa gaaccagcag cagcagcagg gcaataacca cactaatgga actggccacc
     4741 cagggaatca agacagcagt cacacacagg gacccccgtt gaagaaagtg agagttgttc
     4801 ctcctaccac tacctcaggt ggacttatca tgacctcaga ctatcagcgt tccaatccac
     4861 atgctgccta tcccaaccct ggaccaagca catcacagcc gcagagcagc atgggatact
     4921 cagctacctc ccagcagcct ccacagtact cacatcagac acatcggtac tgatctagag
     4981 cggccgcgga tcccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     5041 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     5101 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     5161 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     5221 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     5281 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     5341 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctcacctc
     5401 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     5461 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc
     5521 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggtgagcaa
     5581 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     5641 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     5701 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     5761 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     5821 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     5881 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     5941 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     6001 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     6061 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     6121 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     6181 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     6241 ccgccactcc accggcggca tggacgagct gtacaagtga acgcgtctgg aacaatcaac
     6301 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6361 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6421 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6481 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6541 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6601 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6661 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     6721 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6781 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6841 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag
     6901 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     6961 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     7021 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7081 gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     7141 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     7201 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     7261 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     7321 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     7381 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     7441 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     7501 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     7561 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7621 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7681 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     7741 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac
     7801 attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     7861 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7921 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7981 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8041 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8101 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8161 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8221 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8281 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8341 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8401 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8461 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8521 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8581 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8641 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8701 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8761 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8821 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8881 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8941 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9001 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9061 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9121 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9181 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9241 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9301 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9361 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9421 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9481 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9541 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9601 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9661 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9721 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9781 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9841 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9901 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9961 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
    10021 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    10081 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    10141 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
    10201 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
    10261 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    10321 gtagt
//
