LOCUS       sequence-130304-         518 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) 
            neuronal inhibitor, with IRES-EGFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM
  TITLE     Chemical and genetic engineering of selective ion channel-ligand 
            interactions.
  JOURNAL   Science. 2011 Sep 2;333(6047):1292-6.
  PUBMED    21885782
REFERENCE   2  (bases 1 to 518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-09-2"; volume: "333(6047)"; pages: "1292-6"
COMMENT     Sequence Label: rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR
FEATURES             Location/Qualifiers
     source          1..518
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..33
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    104..137
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      162..171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 taacttcgta taatgtatgc tatacgaagt tatgggtcga tggtgagatc tggactagag
       61 ggtcgatggt gatgcttgga tcaaaatagg aagaatccca atgataactt cgtataaagt
      121 atcctatacg aagttatact agctagtgtt taaacggatc cgccaccatg gactatggcg
      181 gcgctttgtc tgccgtcgga cgcgaacttt tgttcgttac taatcctgtg gtggtgaacg
      241 ggtccgtcct ggtccctgag gatcaatgtt actgtgccgg atggattgaa tctcgcggca
      301 cgaacggcgc tcagaccgcg tcaaatgtcc tgcagtggct tgcagcagga ttcagcattt
      361 tgctgctgat gttctatgcc taccaaacct ggaaatctac atgcggctgg gaggagatct
      421 atgtgtgcgc cattgaaatg gttaaggtga ttctcgagtt cttttttgag tttaagaatc
      481 cctctatgct ctaccttgcc acaggacacc gggtgcag
//
