LOCUS       sequence-13031-e        3666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1137-HQ858681
FEATURES             Location/Qualifiers
     source          1..3666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      140..149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1223..1322)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1339..1358)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1340..1358)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1363..1379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1363..1379)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1481..1500)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1492..2301
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1559..1578)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2448..3036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2937..2956
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3190..3207
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3366..3393)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3485..3571)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3620..3642
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3634..3651
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3635..3651
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgtcccaga ggaaaggcgc catggcggct gcagtgacgg ccagcaagca tcgcgaggac
      181 caggaggcga tgatggtgga gctcaggaga ggcccctgga cgctcgagga agacaacctc
      241 ctcatgaact acattgcttg ccacggcgag ggccgctgga atctcctcgc ccgctgctcc
      301 gggttgaaga ggaccggcaa gagctgccgg ctcaggtggc tcaactacct gaagcccgac
      361 atcaagcgcg gcaacctgac gcctgaggag cagctgctca tcctcgagct ccactccaag
      421 tgggggaaca ggtggtcgag gatagcgcag cacctgccgg ggcgcaccga caacgagatc
      481 aagaactact ggcggacgcg cgtgcagaag caggcgcggc agctgcgggt ggacgccaac
      541 agcgccgtct tccgcgacgc cgtccgctgc tactggatgc cgcgcctgct ggagaagatg
      601 gccgcgcacc accacggcga cggcgagctg ccgccgccac tgcacccctc gcacaacatc
      661 gccggcacgg cctcctcgcg ctcgccgccg gcccacggca gccagcagga ggacccgagt
      721 agtagcgcgc cggcggccag cggccactac ctgcagcagg tcgacccgag cccgagcacg
      781 tcgacgacgt ccgggtcgac gacctcggcc gcgctcccgc cggtgccctg cttctccgaa
      841 tccgaactaa ggtgggtcgt ggaccagtac ggcgccgctt acggttacac cgacgacctc
      901 gacctcgacg gcggcgccgg cgtggtcgac gacgactcgg ctgccgccgc cgcgctggac
      961 agcctcgggc tggacgggct ggacctgggg cccgccgacg gcggcgtgta ctcggacgcg
     1021 acgctgctgg actacctcaa cgccacgtgc gccggcggca ccaccgtgat gacaatgatg
     1081 ggcggcgtcc acggcgccgc gggcggtggc gacggcgact acggtccgtc gccgtggcgg
     1141 acggacgagc tcggccagac ggcgaggaag ctcggcgatc ctcagcatca atggagagga
     1201 ggtatcaagg gtgggcgcgc cgacccagct ttcttgtaca aagttggcat tataagaaag
     1261 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     1321 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1381 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1441 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1501 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1561 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     1621 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1681 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     1741 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     1801 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     1861 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     1921 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     1981 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     2041 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2101 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2161 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2221 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2281 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2341 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     2401 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2461 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2521 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2581 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2641 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2701 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2761 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2821 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2881 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2941 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3001 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3061 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3121 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3181 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3241 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3301 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     3361 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     3421 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     3481 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3541 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     3601 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     3661 gggccc
//
