LOCUS       sequence-130310-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of PSAM-GlyR (L141F,Y115F) neuronal 
            inhibitor, with IRES-EGFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM
  TITLE     Chemical and genetic engineering of selective ion channel-ligand 
            interactions.
  JOURNAL   Science. 2011 Sep 2;333(6047):1292-6.
  PUBMED    21885782
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-09-2"; volume: "333(6047)"; pages: "1292-6"
COMMENT     Sequence Label: rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    96..129
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    200..233
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttctcg agtggatcat ctgcagaatt
       61 cgcccttttc agtgtttcga acctcttcga gggacataac ttcgtataat gtatgctata
      121 cgaagttatg ggtcgatggt gagatctgga ctagagggtc gatggtgatg cttggatcaa
      181 aataggaaga atcccaatga taacttcgta taaagtatcc tatacgaagt tatactaggg
      241 ctagcaagct tgaattcacc atgcgctgtt ctccaggcgg cgtgtggctc gccctggctg
      301 cttcccttct gcacgttagc ctgcagggtg agttccagcg caaactgtat aaggagcttg
      361 ttaagaatta taaccccctg gagcggccgg tcgcaaatga ttcccagcca ctgacagtgt
      421 acttcagcct ctccttgctg cagatcatgg acgtggatga aaagaaccag gtgctgacca
      481 ctaatatttg gttgcagatg tcctggaccg atcactactt gcagtggaat gtgagcgaat
      541 acccaggtgt aaagactgta agattccctg acggccaaat ctggaaacca gatatcctgc
      601 tgttcaacag cgcagacgaa aggtttgatg caacatttca caccaacgtg ttggtcaatt
      661 cttcaggcca ctgccagtac ttcc
//
