LOCUS       sequence-130320-         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid level 1 Alpha plant expression vector. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB3_alpha2R
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(36..53)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(36..52)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(45..67)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(261..280)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    399..423
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 actctagagg atccccgggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt
       61 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      121 agctggcgta atagcgaaga ggcctgcgcc gatcgccctt cccaacagtt gcgcagcctg
      181 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
      241 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
      301 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      361 agacaagctg tgggtctctc tccgcttgag acgatatctg acaggatata ttggcgggta
      421 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
      481 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
      541 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
      601 tcatctgaa
//
