LOCUS       sequence-130322-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express sgRNA and dCas9-KRAB from lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Thakore PI, D'Ippolito AM, Song L, Safi A, Shivakumar NK, Kabadi AM,
            Reddy TE, Crawford GE, Gersbach CA
  TITLE     Highly specific epigenome editing by CRISPR-Cas9 repressors for 
            silencing of distal regulatory elements.
  JOURNAL   Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 
            2015 Oct 26.
  PUBMED    26501517
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3630"; journalName: "Nat Methods"; date: "2015-12"; 
            volume: "12"; issue: "12"; pages: "1143-9"
COMMENT     Sequence Label: pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(32..107)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(124..372)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(182..201)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(352..372)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        405..804
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 gcatacatta tacgaagtta ttaaaaaaaa agcaccgact cggtgccact ttttcaagtt
       61 gataacggac tagccttatt ttaacttgct atttctagct ctaaaactga gacgtccgtc
      121 tccggtgttt cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt
      181 tacggtaagc atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag
      241 aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt
      301 aattccaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg
      361 aaataggccc tcttaattaa ttaacccgtg tcggctccag atctggcctc cgcgccgggt
      421 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
      481 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
      541 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
      601 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
      661 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
      721 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
      781 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg g
//
