LOCUS       sequence-130332-         544 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Module composed of 3 transcriptional units: Luciferase and Renilla 
            proteins, and the Tomato bushy stunt virus (TBSV) silencing 
            suppressor P19 plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB3
            Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos...
FEATURES             Location/Qualifiers
     source          1..544
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(12..356)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(48..68)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 aaaataccgg agtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg
       61 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
      121 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
      181 catctttggg accactgtcg gcaggggcat cttgaacgat agcctttcct ttatcgcaat
      241 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
      301 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
      361 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
      421 ccaccatgtt gacgaagatt ttcttcttgt cattgagtcg taaaagactc tgtatgaact
      481 gttcgccagt cttcacggcg agttctgtta gatcctcgat ctgaattttt gactccatgg
      541 cctt
//
