LOCUS       sequence-130342-         626 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for (human codon optimized) Cas9 plant 
            expression driven by the 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB2alpha2 35s:hCas9:tNos (GB0639)
FEATURES             Location/Qualifiers
     source          1..626
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccttcctctt cttcttgggg tcagccctgc tgtctccacc gagctgagag aggtcgattc
       61 ttgtttcata gagccccgta attgactgat gaatcagtgt ggcgtccagg acctcctttg
      121 tagaggtgta ccgctttctg tctatggtgg tgtcgaagta cttgaaggct gcaggcgcgc
      181 ccaagttggt cagagtaaac aagtggataa tgttttctgc ctgctccctg atgggcttat
      241 ccctgtgctt attgtaagca gaaagcacct tatcgaggtt agcgtcggcg aggatcactc
      301 ttttggagaa ttcgcttatt tgctcgatga tctcatcaag gtagtgtttg tgttgttcca
      361 cgaacagctg cttctgctca ttatcttcgg gagatccttt gagcttttca tagtggctgg
      421 ccagatacaa gaaattaacg tatttagagg gcagtgccag ctcgttacct ttctgcagct
      481 cgcccgcact agcgagcatt cgtttccggc cgttttcaag ctcaaagaga gagtacttgg
      541 gaagcttaat gatgaggtct tttttgacct ctttatatcc tttcgcctcg agaaagtcga
      601 tggggttttt ttcgaagctt gatcgc
//
